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Vol 45, No 4 (2019)

Crop Production

Ecological Stability of Short Stemmed Winter Rye Varieties

Goncharenko A.A., Makarov A.V., Ermakov S.A., Semenova T.V., Tochilin V.N., Tsygankova N.V., Skatova S.E., Krakhmaleva O.A.

Abstract

The results of a 10-year (2008–2017) comparative study of winter rye varieties with recessive-polygenic (the first group) and dominant-monogenic (the second group) short stem type are presented. In each group, five varieties were estimated based on six traits: crop productivity, winter hardiness, plant height, 1000-grain weight, falling number, and viscosity of aqueous extract of grain meal. The following parameters were determined for each trait: phenotypic stability (SF), ecological variation (CV), ecological plasticity (bi), total (TAC) and specific (SAC) adaptive capacity, and breeding value of the genotype (BVG). Average productivity for groups of varieties was identical (6.69 and 6.62 t/ha, respectively); however, short stemmed varieties with dominant-monogenic type significantly differed by the best winter hardiness of plants (for 6.3%) and had a shorter stem (by 9.3 cm). The ecological variation of traits was significantly affected by all three factors: weather conditions of the year (75.5–95.0%), genotype of variety (1.1–2.5%), and variety to year interaction (2.9–22.3%). The year’s weather conditions influenced crop productivity most strongly (86.3% for the first group and 95.0% for the second group). For the winter hardiness trait, the influence of the variety to year factor was higher than for other traits, and it was respectively 9.3 and 22.3%. Ecological stability of the second group varieties was two times higher (CV = 12.4%) than that of the first group varieties (CV = 24.3%). The conclusion that winter rye varieties with a dominant-monogenic short stem had a lower norm of reaction to the adverse conditions of a rewintering developing during years with a high snow cover was made. Therefore, regions where the main limiting factor is low rewintering due to the strong infestation of plants with snow rot have to be the main cultivation area of such winter rye varieties. It is expedient to cultivate different types of short stemmed winter rye varieties in the main winter rye regions of Russia taking into account their agroecological specificity.

Russian Agricultural Sciences. 2019;45(4):315-322
pages 315-322 views

Genotype–Environment Interaction, Productivity, and Adaptive Potential of Spring Wheat Varieties

Sapega V.A.

Abstract

The assessment of productivity and the parameters of adaptability of vetted promising midseason and mid-late varieties of spring wheat under the conditions of the Northern Trans-Ural region was carried out. The varieties were tested in 2014–2016 using fallow as a background in three climatic zones: subtaiga (II zone), northern forest-steppe (III zone), and southern forest-steppe (IV zone). The highest interaction of genotype-environment was noted when calculating the correlation dependence between the productivity of varieties in various years of the test under the conditions of the southern forest-steppe zone (from r = –0.07 ± 0.28 to r = 0.41 ± 0.25). The highest maximum and average productivity according to the assessment of spring wheat varieties in nine environments (3 years × three state crop testing site) was the Aviada variety approved for use (5.86 and 3.78 t/ha, respectively) in the midseason group and promising variety Kvintus (6.98 and 4.58 t/ha) in the mid-late group. Significant productivity variability was noted. In the group of midseason varieties, its value ranged from 23.0 (Ikar) to 29.9% (Aviada), while that in mid-late varieties was from 25.6 (Riks) to 31.6% (Kvintus). The stress resistance of varieties was low regardless of the maturity group. In the group of midseason varieties, the Ikar variety had the highest index of stress resistance (–2.32), while Riks had the highest index in the mid-late group (–2.54). The highest average productivity under contrasting conditions among midseason varieties was observed for the Aviada variety (4.24 t/ha), and that among mid-late varieties was for the Kvintus variety (4.73 t/ha). In terms of responsiveness to changing conditions, three groups of varieties were distinguished: strongly responsive (midseason Lutestsens 70, Aviada, mid-late Melodiya, Kvintus), plastic (midseason Chernyava 13, Skent 3, Omskaya 36, Tyumenskaya 25, Tyumenskaya 29, mid-late Ingala, Yamalskaya) and weakly responsive (midseason varieties Ikar, Sigma, mid-late varieties Riks, Stepnaya Niva). In all varieties, regardless of the ripeness group, low homeostaticity was determined. The Ikar variety (Hom = 6.59) was the best based on homeostaticity in the midseason group, and Riks was the best in the mid-late group (Hom = 5.48). In general, all varieties were characterized by a low general adaptive ability (GAA). Relatively high values of GAA in the midseason group were found for the Aviada variety (GAA = 0.19) and for Kvintus (GAA = 0.50) in the mid-late group. Based on the comprehensive assessment of varieties in terms of productivity and adaptability parameters, Tyumenskaya 25 and Tyumenskaya 29 were the best midseason varieties in the North Trans-Urals, while Melodiya and Ingala were the best among mid-late varieties.

Russian Agricultural Sciences. 2019;45(4):323-329
pages 323-329 views

Dependence of Soybean Productivity on Ecological and Agrochemical Factors

Sinegovskaya V.T., Naumchenko E.T.

Abstract

The results of research into the efficiency of mineral and organic-and-mineral fertilization systems in a long-term, stationary five-field rotation, including soybean and grain crops, are analyzed as dependent on certain agro-ecological factors. The aim of this work was to study the effect of continuous application of mineral (annual N42Р48 per ha of the crop rotation area) and organic-and-mineral (annual N24Р30 + 4.8 t of manure per ha of the crop rotation area) fertilization systems on the productivity of soybean in the crop rotation. The subjects of research were the middle and early ripening varieties of this crop, sown after annual grasses (soybean and oats mixture) and spring wheat. The preceding crop, the variety, and the hydrothermal conditions in 1963–2017 were considered as agro-ecological factors. The highest yield was obtained when sowing soybean crops after wheat: throughout a number of years, the yield of 1.8–2.0 t ha–1 was by 16% more frequent in this case than when soybean was cultivated after annual grasses. The maximum increase against the reference (0.17 t ha–1) was obtained with long-term application of organic and mineral fertilizers to the soybean crop cultivated after wheat. In cold wet years, using such a system of fertilization ensured the plant’s resistance to adverse weather conditions as well as an increase in soybean yield by 0.18 t ha–1 against the reference. The following midseason soybean varieties were the most responsive to fertilization: their grain yield was 0.03–0.20 t ha–1 higher compared to the reference and the potential productivity was fulfilled 2–7% better.

Russian Agricultural Sciences. 2019;45(4):330-333
pages 330-333 views

Complete Study of Nature and Importance of Spectral Bands Contained in Infrared Spectra of Leaves of Maize Inbred Lines with Significant Breeding Properties

Radenović Č.N., Maksimov G.V., Shutova V.V., Slatinskaya O.V., Protopopov F.F., Delić N.S., Grchić N.M., Pavlov J.M., Čamdžija Z.F.

Abstract

In this article we used the method of IR spectroscopy to identify bioactive organic compounds and their structural characteristics in the leaves of maize inbred lines ZPPL 186, ZPPL 225 and M1-3-3 Sdms based on a standard study of high intensity high-amplitude spectral bands and based on attempt-study of hypothetically influential low intensity low-amplitude spectral bands. The made assumption was that numerous spectral bands, not analyzed yet, but occurring in different kinetic forms should be systematically analyzed and the dynamics of their formation caused by different oscillations and vibrations of molecular bonds of organic compounds and their functional groups should be proven. Low intensity spectral bands imply unstable state of the biological system (leaf), which is a consequence of the excited state of molecules, radicals, atoms or ions in tissues, cells or biomembranes. Similar transport processes occur when biological systems are rhythmically stimulated, as well as in the case of a complex ion transport across the excited thylakoid membrane. These bands most frequently occurred in the wave number range of 500–1600 cm–1; they appeared in several spots in wave number ranges up to 3000 cm–1. Amines, primary and secondary amides, proteins, amino acids, alkanes, alkenes, aldehydes, ketones, aromatic compounds, carbohydrates, carboxylic acids, ethers, alcohols, sulfides, mono- and polysaccharides were identified in leaves of inbred lines. The analysis of spectral bands showed that the most intensive was the inbred line ZPPL 186, then the ZPPL M1-3-3 Sdms, while the lowest intensity was detected in ZPPL 225. Thus, by overall study it is possible to identify not only organic compounds and their structures in leaves of inbred lines, but also to indicate the possibility of causing an unstable and conformational state in them.

Russian Agricultural Sciences. 2019;45(4):334-339
pages 334-339 views

Breeding of Spring Turnip Rape in Western Siberia

Kuznetsova G.N., Polyakova R.S.

Abstract

The biological, climatic potential, and soil conditions for territory of Western Siberia are suitable for cultivating cruciferous oil crops (rapeseed and turnip rape). Oil crops are widely used for human nutrition, feeding livestock, many industries, medicine, and perfumery. The oil contains unsaturated fatty acids (oleic, linoleic, and linolenic), which are essential in human nutrition. Increase in productivity is the main goal in turnip rape breeding; much attention is paid to oil increase in seeds and improvements of oil quality (low content of glucosinolates in seeds and the absence of erucic acid in the oil). Brief characteristics of zoned turnip rape varieties are given: productivity (seed yield, oil content, and oil yield) and fatty acid composition of oil. It has been shown that the created varieties have a high adaptability to severe Siberian conditions and resistance to the main environmental factors.

Russian Agricultural Sciences. 2019;45(4):340-343
pages 340-343 views

Mode of Action in Bacteria Bacillus subtilis no. 2 and Humic Preparation on Fruit Formation in Sweet Pepper

Pishchik V.N., Vorobyov N.I., Udalova O.R., Surin V.G., Khomyakov Y.V., Popov A.A.

Abstract

The efficacy of the bacterium Bacillus subtilis no. 2 and humic preparation Stimulife on yield and fruit formation in sweet pepper (Capsicum annum L.) was studied using the technologies with their separate and combined applications. The application of Bacillus subtilis no. 2 was shown to decrease the coefficients of variation in the leaf and fruit masses of pepper plants. The maximum directional effects of the above preparations were revealed by graph analysis. The effects of Bacillus subtilis no. 2 and HP Stimulife are focused on increasing the number of leaves on pepper plants and decreasing the vegetation period of the plants, respectively; their joint effect is focused on increasing the number of fruits on a single plant. Factor analysis has shown that the combined use of Bacillus subtilis no. 2 and HP Stimulife has a negative synergistic effect on chlorophyll (a + b) and growth duration. The varieties of pepper plants exhibited different responses to the above preparations. It has been concluded that the humic preparation, in addition to a direct effect on plants, can also manifest its physiological activity indirectly through the activation of auxin synthesis by PGPR bacteria associatively related to plants.

Russian Agricultural Sciences. 2019;45(4):344-350
pages 344-350 views

Plant Protection

Mycotoxicological Study of Maize Feed Grain (1998–2018)

Kononenko G.P., Burkin A.A., Zotova E.V., Smirnov A.M.

Abstract

For maize grain, which has been important for the forage production of the country over the past two decades, persistent contamination with toxins of fusarium fungi, more often T-2/HT-2 toxins, fumonisins, and more rarely with deoxynivalenol and zearalenone in quantities that pose risks for animal intoxication was showed by enzyme immunoassay. The cases of superintensive accumulation of cyclopiazonic acid, citrinin, mycophenolic acid, and ochratoxin A were revealed. In 2016–2018, for maize grain from four subjects of the Central Federal District of the Russian Federation, a significant prevalence of T-2/HT-2 toxins, deoxynivalenol, and fumonisins with fluctuations in the frequency of occurrence of zearalenone over the years was found; diacetoxyscirpenol was detected in several samples from the Kursk and Voronezh oblasts. Contamination of grain with alternariol in 2016 and 2017 was mild both by frequency (5.3%) and accumulation levels (20–85 μg/kg), but the proportion of samples containing this toxin was 40.7% with a content range of 25–295 μg/kg in the yield of 2018. The influence of soil and climatic factors on the nature of mycotoxin contamination of the maize grain yield, the contribution of fungi belonging to dematiaceous hyphomycetes, and the prevailing variants of combined contamination with fusariotoxins are discussed.

Russian Agricultural Sciences. 2019;45(4):351-355
pages 351-355 views

Phytopathology

The Impact of Pesticides Used in Greenhouses on the Predatory Mite Neoseiulus californicus (Parasitiformes, Phytoseiidae)

Glinushkin A.P., Yakovleva I.N., Meshkov Y.I.

Abstract

Nine commercial pesticides currently used in Russian greenhouses against pests (aphids, thrips, whiteflies) were selected to determine their toxicity to predatory mite Neoseiulus californicus McGregor in order to find out the potential use of acariphage against the background of chemical treatments by Vertimec (abamectin), Fitoverm (aversectin C), Kliper (bifenthrin), Novaction (malation), Oberon (spiromesifen), Bitoxibacillin (Bacillus thuringiensis var. thuringiensis), Actara (tiametoxam), Admiral (piriproxifen), Confidor (imidacloprid), Mospilan (acetamiprid). The recommended concentration of the preparation (T), half the concentration (1/2T), and a double concentration (2T) were used on the female mite in the experiments. The side effects of the pesticides applied to the predator mite were determined 1, 3, and 5 days after the application. The recommended concentration of the preparation of Vertimec, Fitoverm, and Kliper showed a higher toxic effect on N. californicus femails (mortality rate 100%). The obtained data reveals the impossibility of their joint application with the release of N. californicus. The toxic effects of Mospilan, Confidor, Admiral, Actara, Bitoxibacillin, and Novaktion on N. californicus females were low (0–16.6% mortality of females). The present study confirms that the use of predatory mite N. californicus in combination with these pesticides is prospective in integrated pest management in Russian greenhouse conditions.

Russian Agricultural Sciences. 2019;45(4):356-359
pages 356-359 views

Soil Science

Agroecosystem Stability with the Application of Fertilizers and Biopreparations

Alferov A.A., Chernova L.S.

Abstract

Biomass of spring wheat on sod-podzolic soil largely forms due to soil nitrogen, the share of which reaches four-fifths of the total removal of the element when using mineral fertilizers. Inoculation increases the fertilizer nitrogen use efficiency by 4.5% and reduces losses by 7%; there is a trend to increase the immobilization of N fertilizers. Stability of an agroecosystem is characterized by nitrogen flows. During the growing season of spring wheat, the amount of mineralized nitrogen depending on the fertilizer amounts to 17.4–18.0 g/m2, while the amount of reimmobilized nitrogen is 4.4–4.9 g/m2 and net-mineralization (N-M) is 13.1 g/m2. The inoculation of seeds with Rhizoagrin does not significantly affect the processes of mineralization (M) and reimmobilization (RI) in anything but a positive trend of increase in mineralization and reimmobilization of nitrogen in the soil. The use of nitrogen fertilizer brings the agroecosystem into a state of resistance (the maximum threshold limit of exposure (RI : M = 25% and N-M : RI = 3)). On average, over the years of research, inoculation of seeds with Rhizoagrin did not change the indicators of an agroecosystem’s stability with the application of the fertilizers.

Russian Agricultural Sciences. 2019;45(4):360-363
pages 360-363 views

Biological Activity of Sod-Podzolic Soils in a Long-Term Experiment Involving Various Agrotechnical Methods

Kovalevskaya N.P., Zavyalova N.E., Sharavin D.Y., Fomin D.S.

Abstract

The effects from the multiyear use of grain–fallow–plow crop rotation schemes comprising legumes, permanent cultivation of winter rye and barley, and bare fallow regime on the ecological and physiological state of microbial communities in a sod-podzolic heavy loamy soil were examined in the course of a long-term, stationary experiment. Biological activity parameters of soil microbiocoenoses, including the potential intensity of CO2 emission, microbial biomass gross respiration rate, metabolic coefficient, and nitrogen fixation, were assessed. It is shown that the application of organic fertilizers (manure) in the crop rotation scheme with 28.6% legumes ensures the best humus state of the soil and a significant increase in the total nitrogen content. It is established that the share of microbial biomass (Cmic) in the total organic matter of the sod-podzolic heavy loamy soil is 1.50–3.24%. The application of mineral fertilizers results in a slight decrease in the microbial biomass carbon content and increase in the labile organic carbon content. The metabolic activity of microbial communities decreases in all long-term experimental scenarios regardless of the land use techniques. The permanent intensive cultivation of crops, either with or without crop rotation, disturbs the microbiocoenosis stability in soils of the Cis-Ural region.

Russian Agricultural Sciences. 2019;45(4):364-368
pages 364-368 views

Economics

Comparative Analysis into the Efficiency of Agricultural Manufacture of Various Forms of Management

Voitleva Z.A.

Abstract

The article proposes the possibility of solving the urgent problem of increasing agricultural efficiency in Russia by improving the organizational and economic structure of the regions' agrarian economy, suggesting an increase in the number of agricultural producers for those forms of management that demonstrate greater efficiency and a decrease in the number of farms of less efficient forms of management. The article describes the method developed by the author for a comparative, comprehensive analysis of agricultural production’s efficiency in economic entities of various forms of business as well as its use on the example of one of Russia’s regions: the Republic of Adygea. The need for such an analysis at the regional level is emphasized, since Russia’s regions differ in climatic and production-economic conditions, established traditions, living and other conditions of villagers, and, according to the author, these regional features affect the results of the analysis. The results of the analysis allow determining the direction of changes in the existing organizational and economic structure of the agrarian economy in a particular region, which can contribute to the increase in the agricultural production’s efficiency, and to develop appropriate measures by the executive authorities.

Russian Agricultural Sciences. 2019;45(4):369-373
pages 369-373 views

Animal Husbandry

Characteristics of the Romanov Breed by the BMP-15 Locus Responsible for Prolificacy in Sheep

Marzanov N.S., Maluchenko O.P., Koreckaja E.A., Marzanova S.N., Marzanova L.K., Timoshenko Y.I., Feizullaev F.R.

Abstract

The actual problem in identifying a prolificacy gene in Romanov breed sheep is considered. The new diagnostic method to identify the polymorphic gene of the bone morphogenetic protein 15 (BMP-15) influencing prolificacy in the Romanov sheep has been proposed based on the surveys. The frequencies of occurrence of the normal (wild-type) and mutant alleles and the genotypes present at a locus responsible for prolificacy in sheep are revealed. The quantitative indicators, including the numbers of lambs at birth and at weaning and the barren and culled ewes, were recorded with taking into consideration the genotypes at the BMP-15 locus. The correlation between the frequency of occurrence and the number of the produced posterity and among the barren and culled Romanov ewes were ascertained. It was indicated that the highest percentage of the lamb output at birth and at weaning was typical for the ewes homozygous (MM) for a mutant allele. The animals referred to as heterozygous genotypes (WM) appeared in the second position. The highest percentage of the culled and barren ewes was recorded in the carriers of the WW homozygote. Based on the obtained results, it has been concluded that the genotypes at the BMP15 locus should be taken into consideration when selecting the ewes with regard to their fertility. However, further researches into the determined phenomenon in the Romanov sheep are required to improve this performance, since multifetal pregnancies in some WW-group ewes have been registered.

Russian Agricultural Sciences. 2019;45(4):374-377
pages 374-377 views

Effects of Serratula coronata Infusion on Metabolism and Growth Rates in Young Sheep

Zharikov Y.A., Volodina S.O., Volodin V.V., Kaneva L.A.

Abstract

The effects of the leaves of the crown-shaped saw-wort (Serratula coronata L.) used in the water infusion on the young sheep growth rates and serum biochemical profile have been studied. In the context of the deficient protein and excessive dietary fiber intake, a positive tendency for increasing the lamb growth rates dependent on the daily dosage of the crown-shaped saw-wort infusion was revealed. The young stock consuming a 300- and 400-mL infusion a day could increase their live weight over a 2-month period of the experiment by 1.68 kg and 1.86 kg more or 17.9 and 19.9% higher, respectively, than that in the reference animal group, respectively. Increases in the serum albumin level and the albumin : globulin ratio up to 24% and a simultaneous decline in the urea concentration to 28% were registered. It is shown that, under the effects of ecdysteroids, there is a tendency to normalize the blood glucose level in the young sheep as a result of the activation of the gluconeogenesis due to reamination of the amino acids. This has been proven by elevating the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT). The obtained data on the multifaceted metabolic effects of the crown-shaped saw-wort infusion allows us to recommend it as a feed additive improving the growth performance of the young sheep.

Russian Agricultural Sciences. 2019;45(4):378-381
pages 378-381 views

Modern Technologies for Robotic Cow Milking

Kiselev L.Y., Kamalov R.A., Borisov M.Y., Fedoseeva N.A., Sanova Z.S.

Abstract

Increasing milk productivity and obtaining high-quality products are based on targeted breeding, balanced feeding of animals, and compliance with requirements of modern milking and livestock technology, and they represent one of the areas of improving milk production technologies in modern complexes. For efficient robotic milk production, the following indicators of dairy cattle should be taken into account: high milk productivity and milk flow, tightly attached udder, same-sized teats whose lower point should not be below 33 cm from the floor level, a 3 cm minimum distance between the rear nipples and 12.5–30 cm between the front teats, a 1.5–3.5-cm thickness of teats, rear teats that should be located 3 cm lower than the lower part of the udder, a 7 cm minimum distance between the front and rear teats of the udder, and a vertical deviation angle of the teats of no more than 30°. The main reasons for minor losses of milk during robotic milking can be milking of cows that do not have a full-fledged milk-giving reflex or cases when this reflex was inhibited at the beginning of milking as a result of putting the milking arm on the udder teats. It is shown that the milking frequency varies by day periods relatively weakly and decreases to a minimum only in the second half of the night during robotic milking, which is associated with a relatively late distribution of feed mixtures. It is noted that cows of all ages have almost the same number of robotic milkings, which is approximately two times.

Russian Agricultural Sciences. 2019;45(4):382-385
pages 382-385 views

Milk Fatty Acids Estimation by Mid-Infrared Spectroscopy as Proxy for Prediction of Methane Emission in Dairy Cows

Okpara M.O.

Abstract

Ruminant enteric methane (CH4) emission contributes to global warming. Current enteric CH4 measurement techniques, such as whole-animal chambers and tracer gas techniques require complex instrumentation and, thus, are limited in their use. Thus, a simple, robust and inexpensive measurement technique applicable on a large scale to estimate CH4 emission from dairy cattle would therefore be valuable. Milk fatty acid (MFA) composition has been suggested as a means of predicting enteric CH4 output in lactating dairy cattle because of the common biochemical pathways among CH4 and fatty acids in the rumen. A summary of studies that investigated the predictive power of MFA composition for CH4 emission indicated good potential, with predictive power ranging between 47 and 95%. Until recently, gas chromatography (GC) was the principal method used to determine the MFA profile, but GC is unsuitable for routine analysis. This has led to the application of mid-infrared (MIR) spectroscopy. The major advantages of using MIR spectroscopy to predict CH4 emission include its simplicity and potential practical application at large scale. Disadvantages include the inability to predict important MFA for CH4 prediction, and the moderate predictive power for CH4 emission. It may not be sufficient to predict CH4 emission based on MIR alone. Integration with other factors, like feed intake, nutrient composition of the feed, parity, and lactation stage may improve the prediction of CH4 emission using MIR spectra.

Russian Agricultural Sciences. 2019;45(4):386-392
pages 386-392 views

Veterinary

Specifications for Induction of Antistress Effects of Biologically Active Iodine in Chicken Embryos

Indyukhova E.N., Azarnova T.O., Maximov V.I.

Abstract

The biological and biochemical effects induced by transvaginal treatment with biologically active iodine have been examined with and without the reference severe irritant for chicken embryos and chicks, which are considered the most popular model systems for investigation of a drug’s action in veterinary medicine. An increase in the thyroid activity in the experimental group chickens during the first days of their life is reveled and analyzed. Under the impact of the thermal stress factor, the levels of the iodothyronines were elevated. In particular, it was 1.3 times, 20.9%, 23.2%, and 1.3 times higher for total T3, free T3, total T4, and free T4, respectively, in the experimental chickens compared to the control. Under the reference optimization of the thyroid status, the antioxidant defense system in the organisms of the 1-day-old chickens in experimental group two was activated, resulting in the significant increase in the blood serum antioxidant activity, which was 1.3 higher than that in the control group of chickens. This caused a decrease in the levels of the lipids containing the isolated double bonds, the diene conjugates, the triene conjugates, the oxodiene conjugates, and the Schiff bases, whose levels decreased by 8.8%, 1.4 times, 16.3%, 1.5 times, and 1.4 times, respectively. The key aspects of the iodothyronine mechanism of action at the high intensity of the free radical reactions followed by lipid peroxidation are shown in the form of a diagram in Fig. 1. The results of the survey can offer the opportunity to use the drug products based on the chemical element indicated above properly by the veterinarians and health workers. The more that the stressors affect the organism of a bird, the greater is the antioxidant effect exerted by the thyroid hormones.

Russian Agricultural Sciences. 2019;45(4):393-398
pages 393-398 views

Morphological Characteristics for Oocyte-Cumulus Complexes of Bos taurus and Bos indicus Cattle Used for a Variety of Purposes

Rotar L.N., Souza J.F.

Abstract

The technology for the intravital in vitro bovine embryo production utilizes the qualitative and quantitative criteria for the initial population of the viable oocytes to determine the successful development of blastocysts suitable for native embryo transfer or cryopreservation. Therefore, a study of the reproductive potential of Bos taurus and Bos indicus (zebu cattle) cows used for a variety of purposes with the method for oocyte quality and quantity assessment is of scientific and practical interests. The objective of the work is to perform a comparative analysis of the Russian and Brazilian cattle in the yield of the oocyte-cumulus complexes (OCC) and their morphological characteristics for the dairy and beef cattle within populations. The cows were grouped into the Aberdeen-Angus (n = 82), Black-and-White (n = 62), Nelore (n = 149), and Gir (n = 63) cattle breed categories. The oocyte-cumulus complexes obtained by transvaginal puncture of follicles served as the survey materials. The OCC quality was assessed with the use of the following parameters: the presence of OCC layers, the OCC quality, the oocyte size, the homogeneity scale, the ooplasm color, and the turgor pressure. The numbers of the viable OCC of their total quantity comprise 67.2, 42, 83.9, and 71.4% for the Aberdeen-Angus, Black-and-White, Nelore, and Gir cattle breed categories, respectively. It has been concluded that the Bos indicus (zebu-like) cattle have the potential to be used in the technology for in vitro embryo production, since they are more than twice as likely to dominate over the Bos taurus cattle in the oocyte yield.

Russian Agricultural Sciences. 2019;45(4):399-402
pages 399-402 views

Mechanization

Technology for Soil Treatment in Rows of Seedlings and Saplings of Forest and Horticultural Crops

Semenenko S.Y., Abezin V.G., Bespalova O.N., Ageenko O.M.

Abstract

Analysis of the mechanization level in soil treatment for plantings of seedlings and saplings of forest and horticultural crops that is necessary for weeding, keeping soil in a loose state, introducing nutrients into the root layer of the soil, etc. has revealed that interrow treatment has a 100% result. However, the plant rows that include protective zones remain untreated in this case, which requires a considerable amount of manual labor, which is 40–50 people/h per ha to perform the row treatment operation. The best option for the care of planted seedlings for saplings of forest and horticultural crops is the reequipment of the cultivators with a KRN mechanism that allows them to process the soil, simultaneously performing soil-treatment operations both in rows and row spaces. All known methods of tillage in rows require the use of special mechanisms that loosen the soil in a row and cut weeds. Such executive devices must create a necessary protective zone around plants. The developed structure of the mechanism is installed on the KRN row cultivator over the processed row, which simultaneously processes row spaces and rows. The results of the theoretical studies of the developed mechanism are presented.

Russian Agricultural Sciences. 2019;45(4):403-406
pages 403-406 views