Vol 61, No 4 (2025)

Basic human fibroblast growth factor FGF-2 plays a major role in wound healing. Thus, its regenerative potential attracts both researchers and commercial drug manufacturers, including cell therapy developers. This research aims to optimize purification method to obtain high purity rhFGF-2 expressed in methylotrophic yeast Pichia pastoris. Suggested method allows purification of rhFGF-2 with purity of >98% as determined by SDS-PAGE. The effective proliferative dose of rhFGF-2 determined by WST-1 proliferative assay on 3T3 mouse fibroblast cell culture is 5.73 ± 2.16 ng/ml. The presented optimized technique may be attractive for the development of an industrial technology for rhFGF-2 production.

Enzymatic polymerization/copolymerization of aniline (ANI) and 3-aminobenzoic acid (3AB А) was performed using natural polyelectrolyte sodium lignosulfonate (LS) as template. The fungal laccase Trametes hirsuta was used as a catalyst, and air oxygen served as an oxidant. Water-soluble complexes of homopolymers polyaniline (PANI) and poly(3-aminobenzoic acid) ( P ( 3ABA )) and copolymer poly(aniline-co-3-aminobenzoic acid) ( P ( ANI-3ABA )) were prepared and characterized by UV-Vis and FTIR spectroscopy and cyclic voltammetry. The conductivity of the samples was determined. The P(ANI-3ABK)–LS copolymer and P(3ABK)–LS homopolymer complexes showed high efficiency as an active component of UV-blocking films.

The aim of this study was to investigate the effect of naringenin on the growth dynamics of planktonic culture, biofilm density, as well as the concentration of cAMP and pectinase activity of P. syringaei and R. leguminosarum. The studies showed that naringenin did not affect the growth dynamics of the planktonic culture of P. syringaei, but the titer of the R. leguminasarum culture decreased at 1 nM naringenin. 500 pM naringenin suppressed the density of P. syringae biofilms and stimulated it in rhizobia. The cAMP level under the influence of both naringenin concentrations increased to varying degrees both in planktonic and biofilms in both cultures. Naringenin completely suppressed pectinase activity in P. syringaei biofilms, but stimulated it in R. leguminasarum . Thus, naringenin can be considered as an exogenous promising regulator for practical application in the fight against Pseudomonas syringae pv. pisi.

Currently, environmentally friendly processes for processing raw materials containing rare earth elements (REE) are being actively developed. Microorganisms play an important role in the biogeochemistry of REE, but the nature of the interaction of micromycetes with REE remains poorly understood. The study examines the potential of extracting REES from their insoluble forms using microscopic fungi. Using the example of the soil micromycete Aspergillus niger , the possibility of converting difficult-to-dissolve neodymium oxide Nd₂O₃ into water- and alcohol-soluble (ethyl and isopropyl) neodymium compounds is shown. The morpholog y and structure of A. niger cells and the distribution of insoluble and soluble forms of the rare earth element before and after bio-leaching were studied using scanning electron microscopy (SEM). Bio-leaching by micromycetes was modeled using the direct contact method. X-ray fluorescence analysis of extracts after bio-leaching showed the presence of neodymium. These studies will help unlock the potential of microscopic fungi for their application in an environmentally friendly technology for the extraction of REE based on bio-leaching. This may serve as a basis for the development of an environmentally friendly alternative to currently used methods using strong inorganic acids or toxic substances.

The potential of using phage display technology to obtain antigentamycin antibodies has been demonstrated. Antigentamycin recombinant antibodies were obtained for the first time using a sheep phage library (Griffin.1, UK). The interaction between obtained phage antibodies and gentamicin was monitored using a circular dichroism spectroscopy. It was shown that the interaction between antigentamycin phage antibodies and corresponding antibiotic is characterized by the presence of a characteristic exciton doublet: a positive peak at ~233 nm and a more intense negative peak with a maximum at ~240 nm. The possibility of gentamicin indication using a test system based on the dot immunoassay method and antigentamycin recombinant antibodies in aqueous solutions has been demonstrated for the first time; the lower limit of antibiotic detection is 0.5 μ g/ml. Using the dot immunoassay method, it was found that antigentamycin phage antibodies did not exhibit activity towards ampicillin and tetracycline, but showed activity towards kanamycin (lower limit of detection – 1 μ g/ml). The results are promising for further development of methods for gentamicin detection using recombinant phage antibodies.