


Vol 45, No 3 (2019)
- Year: 2019
- Articles: 19
- URL: https://bakhtiniada.ru/1068-3674/issue/view/14298
Crop Production
Adaptive Potential of Winter Wheat’s Source Material in the Middle Volga
Abstract
The results from the assessed adaptability of 14 soft winter wheat varieties of domestic breeding and 29 breeding lines from the Lukyanenko Scientific Center of Agriculture are provided. The stress-resistant varieties with the maximum range of adaptive abilities—Spartak, Marafon, Esaul, Zhemchyuzhina Povolzhya, and L. 351-04 ya0-19-7 line—are marked. The following varieties were characterized by the average yield in contrasting conditions: Vyuga—5.85 t/ha; Severodonetskaya Yubileinaya—5.88 t/ha; Bazis—5.82 t/ha; Zimnitsa—5.62 t/ha; 679 Sv-93 line—6.28 t/ha; and L. 351-04-ya0-19-7 line—6.01 t/ha. The varieties Bazis, Vyuga, and Zimnitsa showed the maximum experimental yield under favorable conditions—7.78, 8.25, and 7.93 t/ha, respectively, which was higher than the yield of the standard Biryuza variety under adverse conditions by 1.16, 0.73, and 0.62 t/ha. According to the drought-tolerance index, varieties Spartak, Severodonetskaya Yubileinaya, Esaul, and Zhemchyuzhina Povolzhya are promising. The falling number of the studied varieties under unfavorable conditions exceeded the norm for wheat of the first class (250–396 s). Under stress conditions, it decreased to 79 s in a number of varieties. The mass fraction of protein and gluten in the grain of most varieties was characterized by low stability. In adverse conditions, the mass fraction of protein in the grain decreased to 10.4% and raw gluten to 17.4%, which is 61.9 and 52.6% of the level of the year favorable for the formation of qualitative grain. The most stable indicators of grain quality are observed in the Biryuza and Vyuga varieties. In adverse conditions, the falling-number was 316 s in Biryuza variety and 395 s in the Vyuga variety. The mass fraction of protein was 14.5 and 14.0%, and raw gluten was 28.2 and 30.0%, respectively.



Genetic Diversity of Barley Accessions from Ethiopia for Powdery Mildew Resistance
Abstract
In laboratory experiments, 925 Ethiopian barley accessions were studied for juvenile resistance to the northwest population of powdery mildew causative agent Blumeria graminis (DC.) Golovin ex Speer f. sp. hordei Marchal. Based on phenotypic screening, the 27 accessions resistant to the pathogen were selected and 47 forms were heterogenous for the studied character. The resistant and susceptible plants of the examined accessions were analyzed with the use of molecular markers developed for identification of the mlo11 allele. Fifteen accessions carrying the mlo11 allele, which determines durable resistance of most modern barley varieties to powdery mildew, were revealed. The accessions differed by the resistance expression that can be explained both by the manifestation of other resistance genes, which are inadequate to the mlo11, and also by the presence of different mlo11 allelic variants in the genotypes of the selected accessions. The resistance to B. graminis of the other 59 forms is controlled by the effective genes that are different from mlo11.



Boiling Soluble Proteins Involved in Drought Stress Adaptation of Embryos and Endosperm of Wheat Cultivars
Abstract
Boiling soluble proteins (BSPs), which constitutes about 0.2% of the total genome, play an key role in stress adaptation. However, drought stress induced regulation of BSPs are still a matter of conjecture. This study primarily focused indicators of oxidative stress like: ROS, lipid peroxidation, injury index and scavenging boiling soluble antioxidant enzymes in embryos and endosperm of different cultivars of wheat under different intensities of drought stress followed by stress recovery. Both in embryos and endosperm of cultivars HD 2967 and PBW 550, MII, MDA and ROS relatively unchanged, minor increased or decreased under severe drought stress conditions. Activities of different boiling soluble antioxidants like BsMDAR, BsTRX.R, BsPAO and BsPDI were substantially higher in the embryos and endosperm of cultivars HD 2967 and PBW 550 under moderate and severe stress conditions, while minor increase or decreased activities were detected in cultivars HD 2851 and HD 2932. Based on present study, it can incidental that cultivars HD2967 and PBW 550 are better equipped to perform biological antioxidant responses to eliminate drought-induced oxidative stress.



Variability of Rice Haploids Obtained From In Vitro Anther Culture
Abstract
Morphological variability of haploid plants and doubled haploids of rice obtained from one callus line in in vitro anther culture has been studied. The study was performed on rice (Oryza sativa L. subsp. japonica Kato) plants of the Kaskad varietу. Regenerants of the same callus line derived from one rice anther (four lines in total) were divided into 2–3 groups of 20–30 plants (depending on the sample size) in the order of their differentiation on a callus and transplantation on the rooting medium. Two callus lines (15.1 and 18.1) generated both haploids and doubled haploids (in equal proportion), while two other callus lines (5.1 and 7.2) formed numerous haploids. In the case of the 5.1 and 7.2 lines, regenerants with a larger ordinal number were characterized by decreased size indices of derived plants (plant height, number of flowers on the main panicle, and the number of panicles). No statistically significant difference has been revealed between the haploids and doubled haploids originating from the 15.1 and 18.1 lines. To induce the doubling of a chromosome number in haploid regenerants by colchicine or other antitubulin compounds, it is recommended to use the earliest plants developing on a callus. Using the Hotelling’s T2 criterion calculated for the whole complex of biometric indices, a statistically significant (p = 0.001) difference was revealed between haploids of four callus lines and doubled haploids of two callus lines. Haploids of different lines differed by 3–4 traits, while doubled haploids differed by three of five traits (panicle length, productive tilling capacity, and plant height). Rice varieties interesting for breeders may be improved using in vitro anther cultures.



Innovation Methods in Molecular Breeding of Sugar Beet (Beta vulgaris L.)
Abstract
Molecular-genetic selection of parental lines of sugar beet has been carried out for further hybridization. Initial lines, including male sterile lines and multigerm pollinators, have been evaluated using 12 microsatellite markers. As a result, two primer pairs have been selected to be the most informative in relation to the heterogeneity of breeding material and the most efficient for a heterosis prediction. A PCR analysis of 12 initial lines of sugar beet has determined specific DNA profiles for breeding material. The performed cluster analysis has allowed us to reveal genetic distances and to divide the studied varieties into clusters depending on their genetic relationship as well as to recommend parental forms for highly productive hybrids. A crossing of genetically distant lines plays an important role in the heterosis manifestation in hybrids. Due to low productivity, combinations of lines whose genetic distance does not exceed 1.41 should be excluded from further analysis. The results of this study are important for the production of heterosis hybrids in the practical breeding of sugar beet.



Growth of Plants and Productivity of Corn in a Cold Climate
Abstract
Under conditions of a relatively cool and humid growing season in the central agro-climatic region of the Komi Republic, corn is not inferior in terms of the growth rate and photosynthetic activity of the crops traditionally cultivated in the northern Nonblack Earth region. The purpose of the research is to study the possibility and prospects of growing early ripening hybrids of corn in the soil and climatic conditions of the Komi Republic. The field experiment was performed on the basis of the Institute of Agriculture of the Komi Scientific Center (Ural Branch, Russian Academy of Sciences) in 2016–2017 on an area of 1.6 ha. Six varieties of corn were studied in 2016 and an additional 15 in 2017. Thus, seven varieties were selected from 21 varieties and hybrids for further study. The results of a preliminary study of the ecological testing of early ripening corn hybrids cultivated under the agro-climatic conditions of the Komi Republic are presented. The features of their growth and development and the formation of green mass yield are considered. Among the studied varieties, the Uralskiy 150 is distinguished with the best feed characteristics. Its plants formed 11–12 leaves with a specific surface density of 0.3 g/dm2, the leaf cenosis index was 4 m2/m, and the maximum rate of photosynthesis of the leaves of the middle layer reached 17 μmol CO2/m2 s. The average yield of green mass of the studied varieties and corn hybrids for two years was 380 kg/ha. The dry matter content in biomass did not exceed 20%, the content of raw protein reached 12%, and the content of fiber reached 29%.



Effect of Selenium Nanoparticles on the Formation of Corn Yield
Abstract
The peculiarities Obsky 140 variety of corn in yield formation when it is cultivated with the use of selenium nanoparticles in an optimum concentration were studied. The experiment was conducted in soil culture in threefold repetition. Agrochemical indicators of soil were: 6.2 pHKCl, 2.6% content of humus, 204 mg/kg P2O5, and 219 mg/kg K2O of soil. Four variants of the experiment were developed: control 1 (dry seeds), control 2 (seeds soaked in water), seed incrustation with NP Se (semidry pickling), and seeds soaked in NP Se (30 min before seeding calculated for 0.1 g/ha). NP Se containing 3.7 mg of Se nanopreparation in 1 L of water was used. The positive effect of selenium nanoparticles on the growth processes of corn seeds—germination, germination energy, and growth force—was shown. Seed treatment with NP Se increased the seed germination energy by 6.90 and 9.13%, laboratory germination by 4.67 and 8.34%, and growth force by 26.32 and 31.05%, respectively, compared with control 2 and control 1. Under adverse conditions, the use of selenium nanoparticles increased plant growth in height. NP Se had a positive effect on starch accumulation, and soaking in this preparation increased the content of protein fractions. The greatest increase in yield of corn’s dry phytomass was provided by the soaking of seeds in selenium nanoparticles, which is caused by an increase corn’s green mass yield.



Water Relation Characteristics of Prunus laurocerasus L. under Progressive Soil Drought Stress of Crimea’s Southern Coast
Abstract
The study examines the ecophysiological response of Prunus laurocerasus L. to the effects of progressive soil drought and determines optimum zones and threshold values of soil moisture, temperature, and light, limiting the photosynthesis and transpiration during its summer active growth period in the southern coast of Crimea. The paper characterizes the development of water stress and photosynthesis inhibition as well as the subsequent recovery after watering. It emphasizes the chlorophyll loss by lamina under the given condition, which was manifested in the yellow-green color of the midrib and brownish spots on the leaves. This leads to the loss of ornamental qualities in the plants. The obtained data on the plant’s functioning mechanisms in response to the environmental effects provides the basis for the ecological assessment of the evergreen species’ physiology and the possibility to choose from a variety of cultivation practices.



Ecology
Environment-Forming and Production Functions of Haloxylon aphyllum in the Karnabchul Desert
Abstract
The environment-forming function of the desert tree black saxaul (Haloxylon aphyllum) in the Karnabchul desert was investigated. As a result, it was found that black saxaul plants of different age had different effects on the degree of illumination. The intensity of solar radiation was mainly affected by middle-aged plants and by old generative specimens to a lesser extent. Black saxaul had a significant effect on the air temperature: it lowered the temperature under the crown and on the crown edge during the daytime, especially at 1:00 p.m.–4:00 p.m., while it increased it in these zones at night. Black saxaul also significantly influenced the temperature of the soil. The temperature was higher at night under the crown, while it was lower than in the outer part of the crown in the daytime. The moisture content of the soil also changed under the influence of black saxaul. Under its crown, the moisture content was noticeably higher than on open natural pastures. It was highest in the upper layers of the soil at the base of the saxaul trunk. As a result of the environment-forming action of black saxaul, favorable hydrothermal conditions for the growth and development of the natural wormwood-ephemeral vegetation under the protection of the shelterbelts and on the pasture areas adjacent to the shelterbelt were formed. The components of the production activity of pasture shelterbelts of black saxaul were shown: fodder mass of saxaul and wormwood-ephemeral vegetation of natural pastures. As a result of the increase in the yield of natural pastures under the protection of the shelterbelts and the harvest of black saxaul, the forage productivity of desert pastures increased more than two times.



Environmental Toxicology of Cadmium and Lead (in the Case of Long Khang, Vietnam)
Abstract
The effects of toxic effects of cadmium and lead on vegetation are revealed. As the experimental culture selected rice, cultivated in the site Long-Khanh in Vietnam. The experimental system included the saturation of soil samples with solutions of toxic metals in 7 concentration levels. As assessment criteria are determined: growth, ramification of rice, the state of the root system, the definition of places of maximum accumulation. The patterns of toxicological effects of lead and cadmium are revealed. Concentration of Cd2+ 0.1 mg/kg stimulates growth, as well as ramification of rice. The concentration of Cd2+ ≥ 30 mg/kg inhibits plant growth. Pb2+ < 10 mg/kg can promote plant growth, increase plant productivity (stimulate very high branched rice, increase root length). At higher concentrations, the tillering tendency tends to decrease. Coefficients of concentrations between pollution levels and estimated state criteria were determined. They characterize the high levels of interconnections from 0.7 to 0.9. Levels of accumulation of heavy metals in parts of plants are revealed. The maximum values are determined in the branches, leaves and roots. The accumulation of Pb in ecosystems is higher than Cd.



Soil Science
Landscape Conditions’ Influence on Soil Properties of Arable Land on a Smooth Slope in a Glaciolacustrine Plain
Abstract
To establish a landscape-focused experiment, the soil cover of an agricultural land unit was studied on a smooth slope of a glaciolacustrine plane, and the factors and parameters of spatial differentiation were determined for several physical, physicochemical, and agrichemical properties. Various degrees of differentiation for certain properties associated with the characteristics of parent rocks were revealed along with the geochemical regimes and the nature of human impact. A significant heterogeneity was recorded in thickness of eluvial horizons, in degree of gleying, soil structure, and organic matter and nitrogen content; moderate heterogeneity was observed in terms of soil texture, physical and chemical properties, and nutritional regime. The main cause for variation of soil properties in the agricultural landscape was the heterogeneity of moraine parent rock: its physical, chemical, and agrochemical properties varied by 19–59%. Natural and human-induced processes in soil formation led to a 2.6-times reduction in the average coefficient of variation for these properties in the arable layer (from 36 to 14%). A number of agrochemical properties became 1.3–1.9 times more heterogeneous due to sheetflood erosion in the transitional eluvial facies, and they became three times more heterogeneous in the eluvial facies as a result of uneven application of organic fertilizers.



Melioration
Substantiation in Selection of Precursors to Rice in Drip Irrigation Conditions on Light Chestnut Soils of the Volga Upland
Abstract
Study results of the All-Russia Research Institute of Irrigated Agriculture obtained in 2014–2016 in conditions of the southern slope of the Volga Upland are presented. The possibility of rice cultivation with innovative water-saving technology on general-purpose irrigation systems, including a different set of agricultural crops as precursors in other crop rotations, has been determined. To assess the preferences of different rice precursors, we selected soybean, a legume culture, potato, a row crop, and a narrow-crop rice. Studies have shown that, despite small time differences, rice crops after all precursors completed the vegetation period and formed a yield close to the planned one without negative impact on soil, phytosanitary condition, and growth and development of rice. All this makes it possible to recommend them to be used as rice precursors in rice crop rotations on general-purpose irrigation systems with periodic irrigation in the following order: soybean → potatoes → rice.



Animal Husbandry
A New Method to Identify Types of Body Constitution in Animals
Abstract
A new method to identify the conformational and constitutional types based on the body specific mass-ratio index (BSMRI) with the use of four live-body measurements and a liveweight in animals has been developed. The cows were assigned to three conformational and constitutional body types by specifying the BSMRI values: flabby (1.36 and less), intermediate (1.37–1.54), and compact (1.55 and more). The objective of the survey is to allocate the Red Steppe cows of the Kubansky intrabreed type to the types of constitution, immunological status, milk production, and reproductive capacity. It is ascertained that the compact type of body condition is typical for the largest portion of cows, comprising 46.2%, which is 11.6 and 27.0% more than that in the specimens ascertained to the intermediate and flabby body condition types, respectively. The milk yields of the cows of a flabby type were, on average, 254–335 kg (P > 0.95) higher than the average milk yield of 5496 kg for the experimental livestock. In addition, the cows assigned to the flabby type produced milk with higher fat- and protein yields and milkability greater by 0.11% (P > 0.99) and 0.09% (P > 0.999) than that in the cows of the compact and intermediate types, respectively. Better welfare was determined in the compact-type cows, which were characterized by a higher production use, when compared to the cows of the other types. Moreover, the cows of a flabby type were inferior to the compact-type animals in the milk yields by 2109 kg on average.



Immunogenetic Characteristics of Trans-Baikal Different-Purpose Sheep
Abstract
The results of the immunogenetic characteristics of Trans-Baikal breed fine-fleece sheep by six blood group systems, including fourteen antigen-specific factors, with respect to the production purposes have been reported. The performed analysis of the immunogenetic parameters of blood revealed similarity in the Bb, Bg, Ca, R, and O antigen-specific factors and remarkable differentiation in the Aa, Ab, Be, and Bi antigens between the wool-and-meat and meat-and-wool sheep. High frequencies of the Bb (0.980), Bi (0.873), Ca (0.932), Ma (0.555), R (0.538), and O (0.910) red blood-cell antigens are typical for the wool-and-meat sheep. The animals with antigens Bd, Be, Bg, and Mb (0.150, 0.278, 0.190, and 0.228, respectively) are rarely found. High concentrations of antigens Bb, Be, Ca, Ma, R, O, and Da (0.960, 0.743, 0.782, 0.812, 0.554, 0.713, and 0.505, respectively) are recorded in meat-and-wool sheep. Low frequencies in occurrence are recorded in the animal carriers of Aa (0.119), Ab (0.109), Bd (0.089), Bi (0.109), Bg (0.129), and Mb (0.149). The Aa, Ab, Bi, Ca, and O antigens are rather often found in the wool-and-meat sheep (P < 0.001), while the meat-and-wool sheep have higher concentrations of Be, Ma, and Da factors (P < 0.001). The genetic identity and distance between the intrabreed specimens comprised 0.652 and 0.428, respectively.



Comparative Evaluation of Yam Peel Meal and Pumpkin Stem waste on the Performance and Carcass Characteristics of Growing Pigs
Abstract
This study was conducted to evaluate the performance and carcass characteristics of growing pigs fed diets supplemented with pumpkin stew waste (PSW) and yam peel meal (YPM). Eighteen (18) crossbred pigs weighed ±26.25 kg were divided into three treatment groups of six (6) per group and replicated thrice with 2 pigs per replicate. The pigs were randomly allocated to three dietary treatment arranged as follows: T1 (basal feed only), T2 (basal feed + 10% PSW), T3 (basal feed + 10% YPM). At the end of 56 days feeding trial, cost implication analysis was carried out. Carcass evaluation was carried out using six Pigs (2 per treatment) which were randomly selected. Results obtained indicated that pigs fed control diet recorded the lowest feed intake (P < 0.05) compared to pigs fed diets supplemented with pumpkin stem waste which recorded the highest significant (P < 0.05) feed intake followed by pigs fed yam peel meal based diet which was not significantly (P > 0.05) different from T1 (Control). Similarly, a significant (P < 0.05) increase in daily weight gain was obtained in pigs fed PSW followed by those fed YPM and those fed Control diet. Final body weight indicated significant (P < 0.05) differences among the treatments with the highest recorded in T3 and the lowest in T1. However, feed conversion ratio, carcass characteristics and cost benefit analysis indicated no significant (P > 0.05) difference among the treatments. These results revealed that supplementing pig diets with kitchen waste such as yam peel meal and pumpkin stem waste could improve growth performance in pigs.



Veterinary
ATPase Activity in Red-Blood and Muscle Tissue Cells in Sheep
Abstract
The objective of the survey is to identify the specificities in the functions of ATPase enzyme systems in blood and the longest back muscle in sheep of two breeds and different ages. Lambs and wethers of Texel and Kuibyshev breeds sampled on the analog principle are the subject of the survey according to the current animal-keeping and feeding standards developed by the Ernst All-Russia Research Institute for Animal Husbandry. It has been ascertained that the activities of Mg2+, Na+, K+-ATPase, Mg2+-ATase, and Na+, K+-ATPase in the lamb’s red-blood cell membranes are determined by the age at high significance levels (P < 0.001) of 99.45, 99.41, and 98.46%, respectively, while their activities in the wethers’ longest back muscle are determined by the age at the levels of 81.09, 85.16, and 83.21%, respectively. The correlation analysis has proven the effects of age on the ATPase activity in the membranes of the red blood cells and the longest back muscle сells. With respect to red blood cells, the correlation is negative, while the muscle tissue is found to have a positive correlation. No significant effect of the breed specificity on the activities of Mg2+, Na+, K+-ATPase, Mg2+-ATPase, and Na+, K+-ATPase in the red-blood and muscle tissue cells was identified.



Physiological Parameters of Thrombocytes in Piglets during the Plant Nutrition Phase in Early Ontogenesis
Abstract
Platelet hemostasis is a very important mechanism of maintaining homeostasis in animals. Its activity level determines the blood rheology in capillaries and the tissue metabolism. Piglets’ growth and development depend on the platelets’ level of functional activity. For this reason, studies into the age-related dynamics of platelet activity in piglets at the end of early ontogenesis are of great importance. It was found that the piglets on plant nutrition showed an increase in the platelets’ aggregation capacity (aggregation of their platelets was accelerated by ADP from 34.1 ± 0.12 s to 27.6 ± 0.12 s, from 24.7 ± 0.08 s to 17.5 ± 0.10 s with collagen, from 36.5 ± 0.08 s to 28.4 ± 0.12 s with thrombin). Intensification of the work of platelets’ receptor and postreceptor mechanisms is the main reason for this effect. The main cause of the revealed increase in platelets’ activity in piglets was the increased thromboxane synthesis (from 48.5 ± 0.05 to 59.8 ± 0.09%). The latter, in turn, was largely due to an increase in the activity of cyclooxygenase (from 78.4 ± 0.06 to 89.8 ± 0.12%) and thromboxane synthetase (from 69.2 ± 0.07 to 81.5 ± 0.07%) and activation of ADP secretion (from 46.0 ± 0.13 to 56.9 ± 0.15%).



Mechanization
Universality of Volatile Corrosion Inhibitors in Terms of Agricultural Production Requirements
Abstract
A qualitative and quantitative system of criteria for evaluating the universality of volatile metal corrosion inhibitors (VCIs) with respect to agricultural production conditions has been developed. The system of qualitative criteria for universalism includes (1) assessment of the effectiveness of metal corrosion inhibition under the conditions of the individual or combined action of corrosion-specific stimulators in the vapor-gas phase; (2) suppression of different types of local corrosion that they cause in the corrosion medium; (3) suppression of the general and local corrosion of contacting-metal structural materials with different compositions under specific conditions; and (4) the observance of acceptable specific characteristics of VCIs: ecological characteristics and characteristics related to the hazard class and influence on chemical oxygen consumption (COC) and biochemical oxygen demand for the biodegradation of substances by microorganisms (BOD5). The system of quantitative criteria for the universalism of VCIs includes quantitative parameters for all proposed qualitative points. It is also proposed to introduce a simplified assessment for agricultural-production conditions by characterizing partially universal inhibitors, which reduces operation costs. Experimental data are given for the IFHAN-114 volatile inhibitor as an example of the effectiveness of the proposed criteria. It is shown that IFHAN-114 is a partially universal inhibitor.



Theoretical Parameters for the Seeding Unit Disk of the Seeder for Hole Sowing of Soybean Seeds
Abstract
To ensure a given rate for seeding of soybean seeds by hole sowing, the sowing disk’s design parameters are presented: the number of cells and the distance between them depending on the diameter of the disk and the linear dimensions of the cell. An analytical relationship was obtained to determine the distance between seeds in a hole and between holes taking into account the central angles between the centers of cells in a group and groups of cells on the side surface of the seeding disk. The required number of cells on the sowing disk and its critical linear velocity were determined taking into account the rate of the seeding unit at which the filling of cells is guaranteed. As a result of the experiment, the following data were obtained: when the diameter of the seeding disk is 0.21 m and the diameter and height of cells are 0.008 m and 0.005 m, respectively, their total number on the side surface of the disk is 120 pcs. The central angle between the centers of cells in the group is 2° and that between the groups of cells is 5°. For the given values of the seeding disk’s design parameters and the given rate of soybeans’ sowing from 9 to 27 pcs./m, the distance between seeds in the hole varies from 0.025 to 0.074 m and that between holes from 0.062 to 0.185 m. This disk is designed and manufactured for laboratory research of the seeding unit for hole sowing of soybean seeds.


