Email this article Comparison of Thermal Stability of New Formate Dehydrogenases by Differential Scanning Calorimetry
- Authors: Pometun A.A.1,2, Kleymenov S.Y.1,3, Zarubina S.A.2,4, Kargov I.S.1,2,4, Parshin P.D.2,4, Sadykhov E.G.1, Savin S.S.2,4, Tishkov V.I.1,2,4
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Affiliations:
- Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”
- Innovations and High Technologies MSU Ltd.
- Koltzov Institute of Developmental Biology
- Department of Chemistry
- Issue: Vol 73, No 2 (2018)
- Pages: 80-84
- Section: Article
- URL: https://bakhtiniada.ru/0027-1314/article/view/163634
- DOI: https://doi.org/10.3103/S002713141802013X
- ID: 163634
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Abstract
Formate dehydrogenases (FDHs) from different sources are systematically studied in our laboratory. Over the past few years, new genes of four FDHs from pathogenic bacterium Staphylococcus aureus (SauFDH), methylotrophic thermotolerant yeast Ogataea parapolymorpha (OpaFDH), yeast Saccharomyces cerevisiae (SceFDH), and moss Physcomitrella patens (PpaFDH) have been cloned and expressed in E. coli cells. By means of differential scanning calorimetry, a comparative study of thermal stability of new recombinant formate dehydrogenases and a number of FDHs from other sources has been performed. It was shown that two new enzymes, SauFDH and OpaFDH, are comparable to the FDH from Pseudomonas sp. 101 bacteria in their stability. SceFDH is the least stable FDH among the described formate dehydrogenases.
About the authors
A. A. Pometun
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”; Innovations and High Technologies MSU Ltd.
Email: vitishkov@gmail.com
Russian Federation, Moscow, 119071; Moscow, 109559
S. Yu. Kleymenov
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”; Koltzov Institute of Developmental Biology
Email: vitishkov@gmail.com
Russian Federation, Moscow, 119071; Moscow, 119334
S. A. Zarubina
Innovations and High Technologies MSU Ltd.; Department of Chemistry
Email: vitishkov@gmail.com
Russian Federation, Moscow, 109559; Moscow, 119991
I. S. Kargov
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”; Innovations and High Technologies MSU Ltd.; Department of Chemistry
Email: vitishkov@gmail.com
Russian Federation, Moscow, 119071; Moscow, 109559; Moscow, 119991
P. D. Parshin
Innovations and High Technologies MSU Ltd.; Department of Chemistry
Email: vitishkov@gmail.com
Russian Federation, Moscow, 109559; Moscow, 119991
E. G. Sadykhov
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”
Email: vitishkov@gmail.com
Russian Federation, Moscow, 119071
S. S. Savin
Innovations and High Technologies MSU Ltd.; Department of Chemistry
Email: vitishkov@gmail.com
Russian Federation, Moscow, 109559; Moscow, 119991
V. I. Tishkov
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”; Innovations and High Technologies MSU Ltd.; Department of Chemistry
Author for correspondence.
Email: vitishkov@gmail.com
Russian Federation, Moscow, 119071; Moscow, 109559; Moscow, 119991
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