电邮这篇文章 Comparison of Thermal Stability of New Formate Dehydrogenases by Differential Scanning Calorimetry
- 作者: Pometun A.A.1,2, Kleymenov S.Y.1,3, Zarubina S.A.2,4, Kargov I.S.1,2,4, Parshin P.D.2,4, Sadykhov E.G.1, Savin S.S.2,4, Tishkov V.I.1,2,4
-
隶属关系:
- Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”
- Innovations and High Technologies MSU Ltd.
- Koltzov Institute of Developmental Biology
- Department of Chemistry
- 期: 卷 73, 编号 2 (2018)
- 页面: 80-84
- 栏目: Article
- URL: https://bakhtiniada.ru/0027-1314/article/view/163634
- DOI: https://doi.org/10.3103/S002713141802013X
- ID: 163634
如何引用文章
开放存取
##reader.subscriptionAccessGranted##
订阅存取
详细
Formate dehydrogenases (FDHs) from different sources are systematically studied in our laboratory. Over the past few years, new genes of four FDHs from pathogenic bacterium Staphylococcus aureus (SauFDH), methylotrophic thermotolerant yeast Ogataea parapolymorpha (OpaFDH), yeast Saccharomyces cerevisiae (SceFDH), and moss Physcomitrella patens (PpaFDH) have been cloned and expressed in E. coli cells. By means of differential scanning calorimetry, a comparative study of thermal stability of new recombinant formate dehydrogenases and a number of FDHs from other sources has been performed. It was shown that two new enzymes, SauFDH and OpaFDH, are comparable to the FDH from Pseudomonas sp. 101 bacteria in their stability. SceFDH is the least stable FDH among the described formate dehydrogenases.
作者简介
A. Pometun
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”; Innovations and High Technologies MSU Ltd.
Email: vitishkov@gmail.com
俄罗斯联邦, Moscow, 119071; Moscow, 109559
S. Kleymenov
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”; Koltzov Institute of Developmental Biology
Email: vitishkov@gmail.com
俄罗斯联邦, Moscow, 119071; Moscow, 119334
S. Zarubina
Innovations and High Technologies MSU Ltd.; Department of Chemistry
Email: vitishkov@gmail.com
俄罗斯联邦, Moscow, 109559; Moscow, 119991
I. Kargov
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”; Innovations and High Technologies MSU Ltd.; Department of Chemistry
Email: vitishkov@gmail.com
俄罗斯联邦, Moscow, 119071; Moscow, 109559; Moscow, 119991
P. Parshin
Innovations and High Technologies MSU Ltd.; Department of Chemistry
Email: vitishkov@gmail.com
俄罗斯联邦, Moscow, 109559; Moscow, 119991
E. Sadykhov
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”
Email: vitishkov@gmail.com
俄罗斯联邦, Moscow, 119071
S. Savin
Innovations and High Technologies MSU Ltd.; Department of Chemistry
Email: vitishkov@gmail.com
俄罗斯联邦, Moscow, 109559; Moscow, 119991
V. Tishkov
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”; Innovations and High Technologies MSU Ltd.; Department of Chemistry
编辑信件的主要联系方式.
Email: vitishkov@gmail.com
俄罗斯联邦, Moscow, 119071; Moscow, 109559; Moscow, 119991
补充文件
