Мақаланы E-mail арқылы жіберу Comparison of Thermal Stability of New Formate Dehydrogenases by Differential Scanning Calorimetry
- Авторлар: Pometun A.A.1,2, Kleymenov S.Y.1,3, Zarubina S.A.2,4, Kargov I.S.1,2,4, Parshin P.D.2,4, Sadykhov E.G.1, Savin S.S.2,4, Tishkov V.I.1,2,4
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Мекемелер:
- Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”
- Innovations and High Technologies MSU Ltd.
- Koltzov Institute of Developmental Biology
- Department of Chemistry
- Шығарылым: Том 73, № 2 (2018)
- Беттер: 80-84
- Бөлім: Article
- URL: https://bakhtiniada.ru/0027-1314/article/view/163634
- DOI: https://doi.org/10.3103/S002713141802013X
- ID: 163634
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Тек жазылушылар үшін
Аннотация
Formate dehydrogenases (FDHs) from different sources are systematically studied in our laboratory. Over the past few years, new genes of four FDHs from pathogenic bacterium Staphylococcus aureus (SauFDH), methylotrophic thermotolerant yeast Ogataea parapolymorpha (OpaFDH), yeast Saccharomyces cerevisiae (SceFDH), and moss Physcomitrella patens (PpaFDH) have been cloned and expressed in E. coli cells. By means of differential scanning calorimetry, a comparative study of thermal stability of new recombinant formate dehydrogenases and a number of FDHs from other sources has been performed. It was shown that two new enzymes, SauFDH and OpaFDH, are comparable to the FDH from Pseudomonas sp. 101 bacteria in their stability. SceFDH is the least stable FDH among the described formate dehydrogenases.
Негізгі сөздер
Авторлар туралы
A. Pometun
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”; Innovations and High Technologies MSU Ltd.
Email: vitishkov@gmail.com
Ресей, Moscow, 119071; Moscow, 109559
S. Kleymenov
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”; Koltzov Institute of Developmental Biology
Email: vitishkov@gmail.com
Ресей, Moscow, 119071; Moscow, 119334
S. Zarubina
Innovations and High Technologies MSU Ltd.; Department of Chemistry
Email: vitishkov@gmail.com
Ресей, Moscow, 109559; Moscow, 119991
I. Kargov
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”; Innovations and High Technologies MSU Ltd.; Department of Chemistry
Email: vitishkov@gmail.com
Ресей, Moscow, 119071; Moscow, 109559; Moscow, 119991
P. Parshin
Innovations and High Technologies MSU Ltd.; Department of Chemistry
Email: vitishkov@gmail.com
Ресей, Moscow, 109559; Moscow, 119991
E. Sadykhov
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”
Email: vitishkov@gmail.com
Ресей, Moscow, 119071
S. Savin
Innovations and High Technologies MSU Ltd.; Department of Chemistry
Email: vitishkov@gmail.com
Ресей, Moscow, 109559; Moscow, 119991
V. Tishkov
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”; Innovations and High Technologies MSU Ltd.; Department of Chemistry
Хат алмасуға жауапты Автор.
Email: vitishkov@gmail.com
Ресей, Moscow, 119071; Moscow, 109559; Moscow, 119991
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