The spectrum of sensitization in children with respiratory allergic diseases living in the Rostov region, according to molecular allergodiagnostics
- Authors: Semernik O.E.1, Lebedenko A.A.1, Sarychev A.M.2, Grebenkina N.A.1, Bakhtin A.V.1, Maltsev S.V.1, Spiridenko G.Y.1
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Affiliations:
- Rostov State Medical University
- Clinical and Diagnostic Center “Health”
- Issue: Vol 21, No 3 (2024)
- Pages: 348-357
- Section: Original studies
- URL: https://bakhtiniada.ru/raj/article/view/268190
- DOI: https://doi.org/10.36691/RJA16928
- ID: 268190
Cite item
Abstract
BACKGROUND: Currently, allergopathology is a global problem all over the world. Due to the widespread occurrence of respiratory allergic diseases, it is of particular interest to study the regional spectrum of sensitization of the population using a modern method-molecular allergodiagnostics. This will help doctors and patients not only identify major allergens, but also competently develop allergen-specific immunotherapy programs.
AIM: To determine the profile (spectrum) of sensitization in children with allergies living in the Rostov region by the method of molecular allergodiagnostics.
MATERIALS AND METHODS: An open multicenter prospective controlled non-randomized study included 67 children with respiratory allergic diseases living in the Rostov region. The patients were examined using the Allergy Explorer 2 (ALEX2) allergy chip. Statistical processing of the results was carried out using the Microsoft Office Excel 2003 and Statistica 12.0 for Windows software package.
RESULTS: According to the conducted studies, it was found that the most common sensitization in children of the Rostov region is caused by weed pollen, pet allergens, pollen of trees, cereals, fruits, mold and yeast fungi, class 4 sensitization was also noted to uteroglobin and lipocalin. Molecular analysis showed that an allergic reaction to ragweed pollen prevailed among pollen allergens ― the allergocomponent Amb a 1 caused sensitization in 27 (40.3%) children, and nettle Ur d ― 23 (34.33%) of the examined. The analysis of the allergen components of tree pollen showed that the most common positive reaction was to date palm (22.39%), cryptomeria japonica (19.4%), while only 6 (8.96%) patients had a “true” allergy to birch pollen (positive component Bet v 1). If we talk about sensitization to house dust mites and mold allergens, it should be noted that the molecules Alternaria alternata (20.89%), Blomia tropicalis (23.88%) and Glycyphagus domesticus (23.88%) prevailed in the spectrum of this group of allergens.
CONCLUSION: In the course of the study, it was found that most often in children of the Rostov region with respiratory allergic diseases, sensitization to weed pollen was determined. This study allowed not only to determine the class of sensitization, but also to identify the allergen components that play a leading role in the development of pathology.
Keywords
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##article.viewOnOriginalSite##About the authors
Olga E. Semernik
Rostov State Medical University
Author for correspondence.
Email: semernick@mail.ru
ORCID iD: 0000-0002-3769-8014
SPIN-code: 1890-5470
MD, Dr. Sci. (Medicine), Assistant Professor
Russian Federation, 29 Nachitsevanskij lane, 344022 Rostov-on-DonAlexander A. Lebedenko
Rostov State Medical University
Email: leb.rost@yandex.ru
ORCID iD: 0000-0003-4525-1500
SPIN-code: 5493-6114
MD, Dr. Sci. (Medicine), Professor
Russian Federation, 29 Nachitsevanskij lane, 344022 Rostov-on-DonAlexey M. Sarychev
Clinical and Diagnostic Center “Health”
Email: sarichevam@gmail.com
ORCID iD: 0009-0007-9041-3077
MD, Cand. Sci. (Medicine)
Russian Federation, 21st Line st., 8344019, Rostov-on-DonNatalia A. Grebenkina
Rostov State Medical University
Email: grebenkinan@bk.ru
ORCID iD: 0000-0002-0842-4399
MD
Russian Federation, 29 Nachitsevanskij lane, 344022 Rostov-on-DonAndrey V. Bakhtin
Rostov State Medical University
Email: pro@bahtin-doc.ru
ORCID iD: 0009-0001-7906-6971
SPIN-code: 4249-4096
MD
Russian Federation, 29 Nachitsevanskij lane, 344022 Rostov-on-DonStanislav V. Maltsev
Rostov State Medical University
Email: steve30@yandex.ru
ORCID iD: 0000-0001-7614-9053
SPIN-code: 7042-7222
MD, Cand. Sci. (Medicine), Assistant Professor
Russian Federation, 29 Nachitsevanskij lane, 344022 Rostov-on-DonGalina Yu. Spiridenko
Rostov State Medical University
Email: Galina.Spiridenko2015@yandex.ru
ORCID iD: 0000-0001-6585-0784
SPIN-code: 8443-9308
MD
Russian Federation, 29 Nachitsevanskij lane, 344022 Rostov-on-DonReferences
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