Email this article Features of lipid peroxidation and antioxidant system status in women after spontaneous miscarriage
- Authors: Ozolinya L.A.1, Bakhareva I.V.1, Overko A.V.1, Nasyrova N.I.2, Argun M.Z.2
-
Affiliations:
- Pirogov Russian National Research Medical University
- Central Clinical Hospital of Civil Aviation
- Issue: Vol 12, No 3 (2025)
- Pages: 343-351
- Section: Original study articles
- URL: https://bakhtiniada.ru/2313-8726/article/view/350247
- DOI: https://doi.org/10.17816/aog646698
- EDN: https://elibrary.ru/KBXUFX
- ID: 350247
Cite item
Open Access
Access granted
Subscription Access
Abstract
Background: Among the complications of miscarriage, the most common are inflammatory conditions, which may be associated with oxidative stress and antioxidant deficiency—a universal mechanism in the development of many conditions, including gynecological diseases.
Aim: The work aimed to investigate lipid peroxidation and antioxidant defense status in patients after first-trimester miscarriage during preventive anti-inflammatory therapy.
Methods: A total of 146 women of reproductive age (26.8±2.8 years) were divided into three groups after miscarriage. Group 1 (n=48) received combined prophylaxis, which consisted of antibacterial therapy (metronidazole±azithromycin) and physiotherapy (magneto–infrared–laser therapy of the pelvic area); Group 2 (n=45) received only physiotherapy; Group 3 (n=53) received only antibacterial therapy. Vaginal microbiome analysis revealed normal and conditionally pathogenic microbiota. Levels of lipid peroxidation (malondialdehyde and diene conjugates) and antioxidant defense system markers (catalase and superoxide dismutase activity) were assessed in all patients and in 30 healthy women, both before treatment and two weeks after therapy.
Results: Compared with healthy women, post-miscarriage patients had a 1.3-fold increase in malondialdehyde levels and a 1.2-fold increase in diene conjugates (p <0.05), as well as a 1.4-fold decrease in catalase and a 1.25-fold decrease in superoxide dismutase (p <0.05). The best outcomes were observed with combined antibacterial and physiotherapy treatment (no complications recorded). The rate of chronic salpingo-oophoritis exacerbation with physiotherapy alone and with antibacterial therapy alone was 4.4% and 5.3%, respectively (p >0.05).
Conclusion: Recovery of the antioxidant system after miscarriage occurs faster with combined therapy including antibacterial drugs and magneto–infrared–laser therapy. This approach significantly reduces the incidence of inflammatory complications.
Full Text
##article.viewOnOriginalSite##About the authors
Lyudmila A. Ozolinya
Pirogov Russian National Research Medical University
Email: ozolinya@yandex.ru
ORCID iD: 0000-0002-2353-123X
SPIN-code: 9407-9014
MD, Dr. Sci. (Medicine)
Russian Federation, 1 Ostrovityanov st, Moscow, 117513Irina V. Bakhareva
Pirogov Russian National Research Medical University
Email: iribakhareva@yandex.ru
ORCID iD: 0000-0002-3078-0744
SPIN-code: 3411-1570
MD, Dr. Sci. (Medicine)
Russian Federation, 1 Ostrovityanov st, Moscow, 117513Alexey V. Overko
Pirogov Russian National Research Medical University
Author for correspondence.
Email: leha.overko@yandex.ru
ORCID iD: 0000-0002-4629-9074
SPIN-code: 5519-2836
Russian Federation, 1 Ostrovityanov st, Moscow, 117513
Nailya I. Nasyrova
Central Clinical Hospital of Civil Aviation
Email: ni_nasyrova@mail.ru
ORCID iD: 0000-0003-0657-7655
SPIN-code: 9016-5566
MD, Cand. Sci. (Medicine)
Russian Federation, MoscowMadina Z. Argun
Central Clinical Hospital of Civil Aviation
Email: www2104@list.ru
ORCID iD: 0000-0003-4841-2496
SPIN-code: 5475-1763
MD, Cand. Sci. (Medicine)
Russian Federation, MoscowReferences
- Sidelnikova VM, Sukhoi GT. Miscarriage of pregnancy: A Guide for practicing physicians. Moscow: Medical Information Agency; 2010. 536 р. (In Russ.) ISBN: 978-5-8948-1813-9
- Gracia C, Sammel M, Chittams J, et al. Risk factors for spontaneous abortion in early symptomatic first-trimester pregnancies. Obstet Gynecol. 2005;106(5 Pt 1):993–999. doi: 10.1097/01.AOG.0000183604.09922.e0
- Dobrokhotova YuE, Sukhoi GT, Ochan TB, et al. The role of hemostatic disorders in the genesis of miscarriage. Russian Journal of Human Reproduction. 2004;10(2):53–54. (In Russ.) EDN: RSVPRB
- Dobrokhotova YuE, Sukhoi GT, Ozerova RI. Undeveloped pregnancy: aspects of etiology. The possibilities of cytokine therapy in the rehabilitation program in the early post-abortion period. Russian Bulletin of Obstetrician-Gynecologist. 2006;(2):13–18. (In Russ.) URL: http://elib.fesmu.ru/Article.aspx?id=145167
- Makarov OV, Ozolina LA, Sumedi TN. Changes in immunity indicators in patients with miscarriage of infectious origin in the first trimester. Russian Bulletin of Obstetrician-Gynecologist. 2008;(6):28–32. (In Russ.) URL: http://elib.fesmu.ru/Article.aspx?id=194192
- Chanchaeva EA, Aizman RI, Gerasev AD. Contemporary perception of antioxidant system of human organism. Ekologiya cheloveka (Human Ecology). 2013;20(7):50–58. doi: 10.17816/humeco17333 EDN: QIYAOR
- Pokalenyeva MSh, Nesterova AM, Sosnova EA, et al. The radical-producing function of neutrophils in habitual miscarriage: the use of the method of activated chemiluminescence with double stimulation. Gynecology, Obstetrics and Perinatology. 2017;16(5):82–88. doi: 10.20953/1726-1678-2017-5-82-88 EDN: ZUKEUF
- Ilyina IYu, Dobrokhotova YuE. Role of oxidative stress in the development of gynecological diseases. Akusherstvo i Ginekologiya. 2021;(2):150–157. doi: 10.18565/aig.2021.2.150-156 EDN: HBFSJC
- Pokaleneva MSh, Nesterova AM, Sosnova EA, et al. Oxidative status of blood plasma in refractory pregnancy loss. V.F. Snegirev Archives of Obstetrics and Gynecology. 2017;4(4:214–219. doi: 10.18821/2313-8726-2017-4-4-214-219 EDN: YPQHDL
- Kotova TV, Iudina MA, Grechkanev GO. Lipid peroxidation and antioxidant defense in patients with bacterial vaginosis and vulvovaginal candidiasis. Russian Bulletin of Obstetrician-Gynecologist. 2013;13(4):31–33. EDN: QZUSUZ
- Nikishov NN, Kalinin BB, Grechkanev GO. The state of lipid peroxidation and antioxidant defense system in patients with chronic pelvic inflammatory diseases. In: 8th All-Russian Scientific Forum "Mother and Child": materials. Moscow; 2006. Р. 465. (In Russ.)
- Hamadyanova AU. Free radical processes in chronic recurrent salpingo-oophoritis and their significance in the selection and control of treatment effectiveness. In: 6th All-Russian Scientific Forum "Mother and Child": materials. Moscow; 2004. Р. 552–553. (In Russ.)
- Churikova MS. The correction of liver functional activity, lipid peroxidation and antioxidant protection of female patients with inflammatory diseases of pelvic organs. Medical Almanac. 2012;(5):74–77. EDN: PGZXCD
- Guo HY, Hu XM, Han DD, et al. Lipid peroxidation and antioxidant status in vagina microenvironment of patients with several common vaginitis. Clin Exp Obstet Gynecol. 2013;40(3):331–336.
- Sosnova EA, Bolevich SB, Pokaleneva MSh. Pathophysiological role of free radical processes in pregnancies end in miscarriage. V.F. Snegirev Archives of Obstetrics and Gynecology. 2016;3(3):136–140. doi: 10.18821/2313-8726-2016-3-3-136-140 EDN: WZHMXZ
- Smirnova DV. Violation of lipid peroxidation in women with chronic endometritis during infertility and the possibility of its correction. In: Biomedical, clinical and social issues of human health and pathology: proceedings of the VIII All-Russian Scientific Conference of Students and Young Scientists with International Participation. Ivanovo; 2022. Р. 7–9. (In Russ.)
- Kolesnikova LI, Danusevich IN, Kurashova NA, et al. Features of lipid peroxidation and antioxidant protection in women with chronic endometritis end reproductive disorders. Fundamental Research. 2013;(9-5):829–832. EDN: RNKOWN
- Isajev AK, Torchinov AM, Umakhanova MM, Chekmareva SE. Magnet-IR-light-laser therapy in the complex treatment of acute inflammatory diseases in the uterine adnexia. Journal of the Russian Society of Obstetricians and Gynecologists. 2004;(4):41–43. (In Russ.)
- Amirkhanova HS, Tsallagova LV, Kabulova IV. Magneto-IR light-laser and ozone therapy in the complex treatment of tubal-peritoneal forms of infertility. Journal of New Medical Technologies. 2019;26(4):58–61. doi: 10.2411/1609-2163-2019-16560 EDN: ZLLBWI
- Clinical guidelines. Miscarriage (spontaneous abortion). 2021–2022–2023 (19.01.2023). (In Russ.)
- Chernyshev IA. Methodical manual on the operation of the magneto-IR-light-laser therapeutic device "Milta-F-5-01". Moscow; 2016. 147 р. (In Russ.)
- Vysokikh MYu, Tyutyunnik VL, Kan NE. Diagnostic significance of malondialdehyde level and catalase activity evaluation in women with preterm labor. Akusherstvo i Ginekologiya. 2017;(4):62–67. doi: 10.18565/aig.2017.4.62-67 EDN: YMREQL
Supplementary files
