The Development of Segmentation Algorithms in Holographic Microscopy and Tomography for Determination of Morphological Parameters of Cells
- 作者: Belashov A.V.1, Gorbenko D.A.1,2, Zhikhoreva A.A.1, Belyaeva T.N.3, Kornilova E.S.3, Semenova I.V.1, Vasyutinskii O.S.1
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隶属关系:
- Ioffe Institute, Russian Academy of Sciences
- ITMO University
- Institute of Cytology, Russian Academy of Sciences
- 期: 卷 45, 编号 11 (2019)
- 页面: 1140-1143
- 栏目: Article
- URL: https://bakhtiniada.ru/1063-7850/article/view/208485
- DOI: https://doi.org/10.1134/S1063785019110191
- ID: 208485
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详细
Algorithms of cell segmentation on two-dimensional phase images and three-dimensional distributions of a refractive index obtained by means of digital holographic microscopy and tomography are developed. The proposed algorithms are optimized for determining cell morphology characteristics including the cell volume, projection area, and surface area. A comparative analysis of the error of cell volume determination by holographic methods using the proposed cell segmentation algorithms and the standard method of confocal fluorescence microscopy has been performed.
作者简介
A. Belashov
Ioffe Institute, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: belashov.andrey.93@gmail.com
俄罗斯联邦, St. Petersburg, 194021
D. Gorbenko
Ioffe Institute, Russian Academy of Sciences; ITMO University
Email: belashov.andrey.93@gmail.com
俄罗斯联邦, St. Petersburg, 194021; St. Petersburg, 197101
A. Zhikhoreva
Ioffe Institute, Russian Academy of Sciences
Email: belashov.andrey.93@gmail.com
俄罗斯联邦, St. Petersburg, 194021
T. Belyaeva
Institute of Cytology, Russian Academy of Sciences
Email: belashov.andrey.93@gmail.com
俄罗斯联邦, St. Petersburg, 194064
E. Kornilova
Institute of Cytology, Russian Academy of Sciences
Email: belashov.andrey.93@gmail.com
俄罗斯联邦, St. Petersburg, 194064
I. Semenova
Ioffe Institute, Russian Academy of Sciences
Email: belashov.andrey.93@gmail.com
俄罗斯联邦, St. Petersburg, 194021
O. Vasyutinskii
Ioffe Institute, Russian Academy of Sciences
Email: belashov.andrey.93@gmail.com
俄罗斯联邦, St. Petersburg, 194021
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