The Development of Segmentation Algorithms in Holographic Microscopy and Tomography for Determination of Morphological Parameters of Cells
- Autores: Belashov A.V.1, Gorbenko D.A.1,2, Zhikhoreva A.A.1, Belyaeva T.N.3, Kornilova E.S.3, Semenova I.V.1, Vasyutinskii O.S.1
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Afiliações:
- Ioffe Institute, Russian Academy of Sciences
- ITMO University
- Institute of Cytology, Russian Academy of Sciences
- Edição: Volume 45, Nº 11 (2019)
- Páginas: 1140-1143
- Seção: Article
- URL: https://bakhtiniada.ru/1063-7850/article/view/208485
- DOI: https://doi.org/10.1134/S1063785019110191
- ID: 208485
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Resumo
Algorithms of cell segmentation on two-dimensional phase images and three-dimensional distributions of a refractive index obtained by means of digital holographic microscopy and tomography are developed. The proposed algorithms are optimized for determining cell morphology characteristics including the cell volume, projection area, and surface area. A comparative analysis of the error of cell volume determination by holographic methods using the proposed cell segmentation algorithms and the standard method of confocal fluorescence microscopy has been performed.
Sobre autores
A. Belashov
Ioffe Institute, Russian Academy of Sciences
Autor responsável pela correspondência
Email: belashov.andrey.93@gmail.com
Rússia, St. Petersburg, 194021
D. Gorbenko
Ioffe Institute, Russian Academy of Sciences; ITMO University
Email: belashov.andrey.93@gmail.com
Rússia, St. Petersburg, 194021; St. Petersburg, 197101
A. Zhikhoreva
Ioffe Institute, Russian Academy of Sciences
Email: belashov.andrey.93@gmail.com
Rússia, St. Petersburg, 194021
T. Belyaeva
Institute of Cytology, Russian Academy of Sciences
Email: belashov.andrey.93@gmail.com
Rússia, St. Petersburg, 194064
E. Kornilova
Institute of Cytology, Russian Academy of Sciences
Email: belashov.andrey.93@gmail.com
Rússia, St. Petersburg, 194064
I. Semenova
Ioffe Institute, Russian Academy of Sciences
Email: belashov.andrey.93@gmail.com
Rússia, St. Petersburg, 194021
O. Vasyutinskii
Ioffe Institute, Russian Academy of Sciences
Email: belashov.andrey.93@gmail.com
Rússia, St. Petersburg, 194021
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