The Development of Segmentation Algorithms in Holographic Microscopy and Tomography for Determination of Morphological Parameters of Cells


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Algorithms of cell segmentation on two-dimensional phase images and three-dimensional distributions of a refractive index obtained by means of digital holographic microscopy and tomography are developed. The proposed algorithms are optimized for determining cell morphology characteristics including the cell volume, projection area, and surface area. A comparative analysis of the error of cell volume determination by holographic methods using the proposed cell segmentation algorithms and the standard method of confocal fluorescence microscopy has been performed.

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A. Belashov

Ioffe Institute, Russian Academy of Sciences

编辑信件的主要联系方式.
Email: belashov.andrey.93@gmail.com
俄罗斯联邦, St. Petersburg, 194021

D. Gorbenko

Ioffe Institute, Russian Academy of Sciences; ITMO University

Email: belashov.andrey.93@gmail.com
俄罗斯联邦, St. Petersburg, 194021; St. Petersburg, 197101

A. Zhikhoreva

Ioffe Institute, Russian Academy of Sciences

Email: belashov.andrey.93@gmail.com
俄罗斯联邦, St. Petersburg, 194021

T. Belyaeva

Institute of Cytology, Russian Academy of Sciences

Email: belashov.andrey.93@gmail.com
俄罗斯联邦, St. Petersburg, 194064

E. Kornilova

Institute of Cytology, Russian Academy of Sciences

Email: belashov.andrey.93@gmail.com
俄罗斯联邦, St. Petersburg, 194064

I. Semenova

Ioffe Institute, Russian Academy of Sciences

Email: belashov.andrey.93@gmail.com
俄罗斯联邦, St. Petersburg, 194021

O. Vasyutinskii

Ioffe Institute, Russian Academy of Sciences

Email: belashov.andrey.93@gmail.com
俄罗斯联邦, St. Petersburg, 194021

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