The Development of Segmentation Algorithms in Holographic Microscopy and Tomography for Determination of Morphological Parameters of Cells


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Resumo

Algorithms of cell segmentation on two-dimensional phase images and three-dimensional distributions of a refractive index obtained by means of digital holographic microscopy and tomography are developed. The proposed algorithms are optimized for determining cell morphology characteristics including the cell volume, projection area, and surface area. A comparative analysis of the error of cell volume determination by holographic methods using the proposed cell segmentation algorithms and the standard method of confocal fluorescence microscopy has been performed.

Sobre autores

A. Belashov

Ioffe Institute, Russian Academy of Sciences

Autor responsável pela correspondência
Email: belashov.andrey.93@gmail.com
Rússia, St. Petersburg, 194021

D. Gorbenko

Ioffe Institute, Russian Academy of Sciences; ITMO University

Email: belashov.andrey.93@gmail.com
Rússia, St. Petersburg, 194021; St. Petersburg, 197101

A. Zhikhoreva

Ioffe Institute, Russian Academy of Sciences

Email: belashov.andrey.93@gmail.com
Rússia, St. Petersburg, 194021

T. Belyaeva

Institute of Cytology, Russian Academy of Sciences

Email: belashov.andrey.93@gmail.com
Rússia, St. Petersburg, 194064

E. Kornilova

Institute of Cytology, Russian Academy of Sciences

Email: belashov.andrey.93@gmail.com
Rússia, St. Petersburg, 194064

I. Semenova

Ioffe Institute, Russian Academy of Sciences

Email: belashov.andrey.93@gmail.com
Rússia, St. Petersburg, 194021

O. Vasyutinskii

Ioffe Institute, Russian Academy of Sciences

Email: belashov.andrey.93@gmail.com
Rússia, St. Petersburg, 194021

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