The influence of modulation of intestinal microbiota on clinical and immunological parameters and oxytocin levels in children with autism spectrum disorders
- Authors: Cherevko N.A.1,2, Novikov P.S.1,2, Khudyakova M.I.1, Arkhipov A.M.2, Loginova E.A.1, Vekovtsev A.A.3, Bylin P.G.3
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Affiliations:
- Siberian State Medical University
- Medical Association “Center for Family Medicine”
- Scientific and Production Association “ArtLife”
- Issue: Vol 27, No 4 (2024)
- Pages: 831-838
- Section: SHORT COMMUNICATIONS
- URL: https://bakhtiniada.ru/1028-7221/article/view/267858
- DOI: https://doi.org/10.46235/1028-7221-16873-TIO
- ID: 267858
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Abstract
Autism spectrum disorders are associated with an imbalance of immune and neurological disorders, starting after the age of two. The study is devoted to studying the role of specialized strains of bacteria Lactobacillus reuteri, which mediate the synthesis of oxytocin in humans and influence inflammation indicators. Bacteria of this strain were part of the biologically active additive “Panbiolact Mental”, developed and presented by NPO ArtLife (Tomsk). The purpose of the work was to assess the effect of specialized strains of bacteria Lactobacillus reuteri on changes in the composition of the intestinal microbiota, oxytocin levels, and immune parameters of children with ASD. The study included 43 children with autism spectrum disorders who took Panbiolact Mental for 90 days. The study materials included venous blood samples and fecal samples. The concentrations of cytokines (IL-4, IL-10, TNF, IFN), immunoglobulins (IgE, IgG, IgA, IgM) and the neuropeptide oxytocin were determined in the blood serum. Fecal samples were used to assess the qualitative and quantitative composition of the colon microbiota. Clinical symptoms of the disease associated with quality of life were assessed using the standard ATEC test scale (Autism Treatment Evaluation Checklist), expressed in scores corresponding to the severity of clinical and neurological parameters of the disease. In children with autism spectrum disorders, after 90 days of regular use of Panbiolact Mental, the number of bacteria of the genera Acinetobacter decreased, the number of Bacteroides species pluralis, Akkermansia muciniphila, Eubacterium rectale, Prevotella species pluralis and Methanobrevibacter smithii increased. Increases in the concentration of oxytocin, the protolerogenic coefficient IL-10/TNFα, immunoglobulins M and G, and a decrease in the concentrations of TNFα and IL-10 were recorded. The results of the study support the hypothesis of a significant role of gut microbiota diversity in the neuro-immune pathogenesis of autism spectrum disorders. “Panbiolact Mental” is presented as a potentially effective remedy for an integrated approach to the correction of ASD in children. These data may form the basis for further research in the field of probiotic therapy, as well as for the development of new strategies based on modulation of the intestinal microbiota.
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##article.viewOnOriginalSite##About the authors
N. A. Cherevko
Siberian State Medical University; Medical Association “Center for Family Medicine”
Email: Pavel.N1234@yandex.ru
PhD, MD (Medicine), Professor, Immunology and Allergy Department, Siberian State Medical University; Allergist-Immunologist, Co-director, Medical Association “Center for Family Medicine”
Russian Federation, Tomsk; TomskP. S. Novikov
Siberian State Medical University; Medical Association “Center for Family Medicine”
Author for correspondence.
Email: Pavel.N1234@yandex.ru
PhD Applicant, Department of Immunology, Siberian State Medical University; Head, Clinical Diagnostic Laboratory, Medical Association “Center for Family Medicine”
Russian Federation, Tomsk; TomskM. I. Khudyakova
Siberian State Medical University
Email: Pavel.N1234@yandex.ru
Applicant, Department of Immunology
Russian Federation, TomskA. M. Arkhipov
Medical Association “Center for Family Medicine”
Email: Pavel.N1234@yandex.ru
Clinical Laboratory Diagnostics Doctor
Russian Federation, TomskE. A. Loginova
Siberian State Medical University
Email: Pavel.N1234@yandex.ru
5th year Student of the Faculty of Medicine
Russian Federation, TomskA. A. Vekovtsev
Scientific and Production Association “ArtLife”
Email: Pavel.N1234@yandex.ru
PhD (Medicine), Director for Science and Production
Russian Federation, TomskP. G. Bylin
Scientific and Production Association “ArtLife”
Email: Pavel.N1234@yandex.ru
Leading Engineer for the Implementation of New Equipment and Technologies
Russian Federation, TomskReferences
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