The effect of stem cells on the functional state of liver tissue against the background of a liver cirrhosis model (experimental study)
- Authors: Kotkas I.Е.1, Mazurov V.I.2
-
Affiliations:
- North-Western State Medical University named after I.I. Mechnikov
- North-Western State Medical University named after I.I. Mechnikov,
- Issue: Vol 12, No 4 (2020)
- Pages: 25-32
- Section: Original research
- URL: https://bakhtiniada.ru/vszgmu/article/view/43911
- DOI: https://doi.org/10.17816/mechnikov43911
- ID: 43911
Cite item
Abstract
Relevance. Treatment of liver cirrhosis is an extremely important problem of modern medicine. Improvement of liver function in this category of patients is important not only for hepatology, but also for surgery, since surgical interventions on the liver in this pathology are often accompanied by the development of liver failure.
Purpose. To evaluate the effect of cell therapy on liver function in the experiment.
Materials and methods. The article presents the results of the experimental use of use of stem cells in simulated liver cirrhosis. The experiment was performed on 132 female c57black mice, which were between 12 and 18 weeks old. After forming a model of liver cirrhosis, in order to assess the effect of cell therapy on the function of liver tissue, individuals were injected with stem cells through the vessels of the peripheral bed and intraportally. 30 days after cell therapy, the blood levels of ALT, AST, alkaline phosphatase, plasma diene conjugants, plasma malondialdehyde, plasma superoxide dismutase, and glutathione peroxidase were evaluated.
Conclusion. According to the findings, obtained in the experiment, the use of cell therapy against the background of simulated cirrhosis of the liver contributed to a decrease in the severity of cytolytic and cholestatic syndromes, stimulation of liver protein function, suppression of free radical oxidation and stimulation of the antioxidant system. At the same time, the best effect was achieved when the cell structures were introduced not into the peripheral vessels, but directly into the vascular bed of the liver.
Full Text
##article.viewOnOriginalSite##About the authors
Inna Е. Kotkas
North-Western State Medical University named after I.I. Mechnikov
Author for correspondence.
Email: inna.kotkas@yandex.ru
ORCID iD: 0000-0003-4605-9887
SPIN-code: 1853-8825
зав. хирургическим отделением, к.м.н., доцент кафедры факультетской хирургии им. И. И. Грекова
Russian Federation, Saint PetersburgVadim I. Mazurov
North-Western State Medical University named after I.I. Mechnikov,
Email: maz.nwgmu@yandex.ru
ORCID iD: 0000-0002-0797-2051
SPIN-code: 6823-5482
Scopus Author ID: 16936315400
head of the department of therapy, rheumatology, examination of temporary disability and the quality of medical care. E.E. Eichwald, w.s. RF, Academician of the Russian Academy of Sciences, MD Professor
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