Focus on the anti-inflammatory effect of glucocorticosteroids in experimental acute lung injury
- 作者: Voloshin N.I.1, Zamiralova Y.A.1, Pugach V.A.2, Salukhov V.V.1, Tyunin M.A.2, Kharitonov M.A.1, Odintsova I.A.1, Slizhov P.A.1, Minakov A.A.1, Slutskaya D.R.1
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隶属关系:
- Military Medical Academy
- State Scientific Research Test Institute of the Military Medicine
- 期: 卷 42, 编号 2 (2023)
- 页面: 167-176
- 栏目: Original articles
- URL: https://bakhtiniada.ru/RMMArep/article/view/316031
- DOI: https://doi.org/10.17816/rmmar456457
- ID: 316031
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AIM: Assessment of the effect of various doses of dexamethasone as an inflammation modulator in experimental lipopolysaccharide-induced acute lung injury in rats.
MATERIALS AND METHODS: Acute lung injury in rats was modeled by intratracheal administration of cell wall lipopolysaccharide from the Salmonella enterica. White male rats were divided into groups: a group of intact animals (n = 10); the control group (n = 40), in which the animals were simulated acute lung injury without further treatment and removed from the experiment on day 3; three experimental groups (n = 40), in which, 3 hours after modeling acute lung injury, and then daily once a day for 3 days, dexamethasone solution was administered intraperitoneally in the following doses: in group 1 — 0.52 mg/kg (equivalent to 6.0 mg/day for a person), in group 2 — 1.71 mg/kg (20.0 mg/day for a person), in group 3 — 8.0 mg/kg (94.0 mg/day, pulse therapy for humans). On the 3rd day, blood samples were taken from the caudal vena cava in surviving animals for clinical analysis and evaluation of the function of mitochondria of peripheral blood leukocytes. To determine the severity of local inflammatory reactions and pulmonary edema, bronchoalveolar lavage was performed with the study of an endopulmonary cytogram and an assessment of pathomorphological changes in the lung tissue.
RESULTS: indicate that dexamethasone reduces the amount of lung tissue damage and animal mortality, dose-dependently reduces the functions of mitochondria and the number of lymphocytes and monocytes in peripheral blood, as well as neutrophils, lymphocytes and macrophages in bronchoalveolar lavage samples.
CONCLUSION: The use of dexamethasone at a dose of 0.52 mg/kg (equivalent to 6.0 mg/day for humans) is accompanied by better survival, minimal effect on the viability and functional activity of inflammatory cells. Pulse therapy leads to a significant decrease in the number of immunocompetent cells in bronchoalveolar lavage, mitochondrial dysfunction in the form of a decrease in the ability of these cells to use the reserve power of mitochondrial respiration in response to the action of a stress factor. Excessive inhibition of immunocompetent cells can contribute to the activation of latent and opportunistic infections, which must be taken into account when choosing a dosing regimen for glucocorticosteroids.
作者简介
Nikita Voloshin
Military Medical Academy
编辑信件的主要联系方式.
Email: nikitavoloshin1990@gmail.com
ORCID iD: 0000-0002-3880-9548
SPIN 代码: 6061-4342
Scopus 作者 ID: 57926549500
Researcher ID: HSG-7925-2023
M.D., postgraduate student of Therapy of Doctors Improvement Department
俄罗斯联邦, Saint PetersburgYuliya Zamiralova
Military Medical Academy
Email: yulia_nikolaeva@hotmail.com
ORCID iD: 0009-0000-5631-9932
SPIN 代码: 7692-9410
Researcher ID: HSG-9775-2023
student
俄罗斯联邦, Saint PetersburgVictoriya Pugach
State Scientific Research Test Institute of the Military Medicine
Email: gniiivm_7@mil.ru
ORCID iD: 0000-0003-4290-350X
SPIN 代码: 3739-3699
Scopus 作者 ID: 57195201128
Researcher ID: ACD-0418-2022
Ph.D. (Biology), Senior researcher
俄罗斯联邦, Saint PetersburgVladimir Salukhov
Military Medical Academy
Email: vlasaluk@yandex.ru
ORCID iD: 0000-0003-1851-0941
SPIN 代码: 4531-6011
Scopus 作者 ID: 55804184100
M.D., D.Sc. (Medicine), Associate Professor, the Head of Therapy of Doctors Improvement the first Department
俄罗斯联邦, Saint PetersburgMikhail Tyunin
State Scientific Research Test Institute of the Military Medicine
Email: gniiivm_7@mil.ru
ORCID iD: 0000-0002-6974-5583
SPIN 代码: 6161-7029
Scopus 作者 ID: 25032311600
M.D., Ph.D. (Medicine), deputy Head of the center
俄罗斯联邦, Saint PetersburgMikhail Kharitonov
Military Medical Academy
Email: micjul11@yandex.ru
ORCID iD: 0000-0002-6521-7986
SPIN 代码: 7678-2278
Scopus 作者 ID: 57521284600
Researcher ID: H-6056-2015
M.D., D.Sc. (Medicine), Professor, the Therapy of Doctors Improvement the first Department
俄罗斯联邦, Saint PetersburgIrina Odintsova
Military Medical Academy
Email: odintsova-irina@mail.ru
ORCID iD: 0000-0002-0143-7402
SPIN 代码: 1523-8394
Scopus 作者 ID: 6603745712
Researcher ID: 96874
D.Sc. (Biology), Professor, the Head of the Histology with a Course in Embryology Department
俄罗斯联邦, Saint PetersburgPavel Slizhov
Military Medical Academy
Email: maidel@bk.ru
ORCID iD: 0000-0001-6885-5273
SPIN 代码: 3626-6262
Scopus 作者 ID: 57203389614
Researcher ID: X-5497-2018
junior researcher of the Department (experimental medicine) of the Research Center
俄罗斯联邦, Saint PetersburgAleksey Minakov
Military Medical Academy
Email: minacom@mail.ru
ORCID iD: 0000-0003-1525-3601
SPIN 代码: 5344-7883
Scopus 作者 ID: 57926549400
Researcher ID: HSG-9445-2023
M.D., postgraduate student of Therapy of Doctors Improvement the first Department
俄罗斯联邦, Saint PetersburgDina Slutskaya
Military Medical Academy
Email: dina_hanieva@mail.ru
ORCID iD: 0000-0003-3910-2621
SPIN 代码: 2546-9393
Researcher ID: 882535
Ph.D. (Biology), Associate Professor, the teacher of Histology with a Course in Embryology Department
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