电邮这篇文章 MICROTEST FOR THE DETERMINATION OF THE AMYLOLYTIC ACTIVITY OF SALIVA ALPHA-AMYLASE
- 作者: Godovalov A.P1, Yakovlev M.V1, Zadorina I.I1
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隶属关系:
- Acad. E.A. Wagner Perm State Medical University
- 期: 卷 23, 编号 3-4 (2019)
- 页面: 115-117
- 栏目: Articles
- URL: https://bakhtiniada.ru/1728-2802/article/view/43028
- DOI: https://doi.org/10.18821/1728-2802-2019-23-3-4-115-117
- ID: 43028
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全文:
详细
It is known that the concentration of alpha-amylase in saliva can determine its catalytic activity, the decrease of which occurs during various pathological processes in the oral cavity. The overwhelming number of methods for determining the catalytic activity of enzymes involve the use of a large volume of reagents and samples, which makes it difficult to study saliva in large groups. The purpose of the study is to evaluate the possibility of using the microvariation of the reaction to determine the activity of saliva alpha-amylase, as well as to analyze the dependence of the enzyme activity on its concentration. Materials and methods. Saliva was obtained from 15 people with intact periodontal disease and the dentition, without somatic pathology. For in vitro studies, alpha-amylase solutions were prepared with an enzyme concentration of 10; five; 2.5; one; 0.5 and 0.25 mg / ml ex tempore. To save samples and reagents, the volume of the reaction participants was proportionally reduced. The further analysis procedure was carried out according to the instructions of the manufacturer of the «AMYLASE-VITAL» reagent kit to determine the activity of alpha-amylase. Statistical analysis of the results was performed using the Student’s t-test in the program Statistica 7.0. Results. The comparability of the results of determining the activity of alpha-amylase using the classical and microplate variants of the reaction is shown. With an increase in alpha-amylase concentration from 0 to 2.5 mg / ml, a directly proportional increase in enzyme activity is observed. In the case of an increase in the concentration of alpha-amylase above 2.5 mg / ml, a decrease in its activity is shown, which may be due to the precipitation of a part of the enzyme. The activity of the enzyme in saliva of practically healthy individuals using the microvariation of the reaction was 528.6 ± 2.4 U / l. In conclusion the use of a microvariant of the reaction for determining the activity of alpha-amylase may be justified for a large number of subjects. A linear dependence of the enzyme activity on its concentration in the range of 0-2.5 mg / ml is shown.
作者简介
A. Godovalov
Acad. E.A. Wagner Perm State Medical University614000, Perm, Russia
M. Yakovlev
Acad. E.A. Wagner Perm State Medical University
Email: mikhailyak@mail.ru
студент стоматологического акультета ФГБОУ ВО «Пермский государственный медицинский университет им. акад. Е.А. Вагнера» Минздрава России 614000, Perm, Russia
I. Zadorina
Acad. E.A. Wagner Perm State Medical University614000, Perm, Russia
参考
- Feng Y., Li Q., Chen J., Yi P., Xu X., Fan Y., et al. Salivary protease spectrum biomarkers of oral cancer. Int J. Oral Sci. 2019;11(1): 7.
- Zhang Q., Chen B., Zhu D., Yan F. Biomarker levels in gingival crevicular fluid of subjects with different periodontal conditions: A cross-sectional study. Arch. Oral Biol. 2016; 72: 92-8.
- Максутова В.О., Цветков В.О., Шпирная И.А., Ибрагимов Р.И. Определение активности амилолитических ферментов по гидролизу субстрата, иммобилизованного в полиакриламидном геле. Доклады Башкирского университета, 2017; 295): 736-40.
- Садовничий В.А., Ветров Д.П., Вишневский В.В. и др. Математический метод определения каталитической активности ферментов в сложных биологических растворах. Интеллектуальные системы. Теория и приложения (ранее: Интеллектуальные системы по 2014, № 2, ISSN 2075-9460). 2013; 17(1-4. - С.): 517-22.
- Яковлев М.В., Батог К.А. Изучение антибиопленочной активности альфа-амилазы. Материалы XVII научной конференции молодых ученых и специалистов с международным участием «Молодые ученые - медицине». Владикавказ; 2018: 339-41.
- Мельник К.Н., Баишева Г.М., Гильмиярова Ф.Н., Аллатова Т.А. Саливадиагностика как метод определения иммунологической адаптации к учебному стрессу в условиях различного питьевого поведения. Клиническая лабораторная диагностика. 2018; 63(6): 353-7.
- Биохимия ротовой жидкости в норме и при патологии. Учебно-методическое пособие. Под ред. Шестопалова А.В. /ФГБОУ ВО РНИМУ имени Н.И. Пирогова Минздрава России. М.: ИКАР; 2017.
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