电邮这篇文章 Sensitivity of pathogenic and opportunistic microorganisms to probiotic strains in the light of personalized probiotic therapy
- 作者: Parakhina A.I.1, Parakhina L.I.1, Troshina D.A.2, Levina K.E.2, Pimenova Y.A.1, Koteneva E.N.1, Evstropov A.N.1, Zakharova L.N.1, Mironov A.Y.3,4, Suranova T.G.4
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隶属关系:
- Novosibirsk State Medical University
- Center of Hygiene and Epidemiology in the Novosibirsk Region
- Moscow Research Institute of Epidemiology and Microbiology named after G.N. Gabrichevsky
- Academy of Postgraduate Education of the Federal Scientific and Clinical Center for Specialized Types of Medical Care and Medical Technologies
- 期: 卷 27, 编号 5 (2022)
- 页面: 280-289
- 栏目: Original study articles
- URL: https://bakhtiniada.ru/1560-9529/article/view/134017
- DOI: https://doi.org/10.17816/EID117545
- ID: 134017
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详细
BACKGROUND: Resistance of microorganisms to antimicrobial drugs (AMP) is one of the global problems of modern medicine, which poses a serious threat to human health. The formation of the resistance of pathogens to AMP forces us to look for new ways to combat them. Probiotic therapy is one of the promising and safe methods of treating many infectious diseases.
AIM: This study assesses the sensitivity of clinical strains of pathogenic and opportunistic bacteria and fungi to microorganisms that are part of probiotic preparations.
MATERIALS AND METHODS: The sensitivity of 93 cultures of pathogenic and opportunistic microorganisms (UPM) isolated from the feces of patients with intestinal dysbiosis to strains of probiotic bacteria isolated from commercial probiotic preparations was studied: Lactobacillus plantarum 8RA-3, Escherichia coli M-17, Bacillus subtilis strain VKPM B-10641, Enterococcus faecium SF-68, Saccharomyces boulardii CNCM I-745 by the delayed antagonism method.
RESULTS: Pathogenic microorganisms showed low and moderate sensitivity to the probiotic strain of L. plantarum. Sensitivity to the probiotic strain of B. subtilis was detected in Staphylococcus aureus strains, low or completely absent in Pseudomonas aeruginosa, Klebsiella spp., Serratia marcescens. The most sensitive to the probiotic strain were clinical strains of E. coli.
CONCLUSION: UPM and pathogenic microorganisms isolated from the gastrointestinal tract of patients with dysbiosis have different sensitivity to probiotic strains: from high to several probiotics to its complete absence. The empirical approach to prescribing a probiotic does not guarantee a therapeutic effect, which makes it prudent to individualize the selection of a probiotic based on an assessment of its antimicrobial activity against isolated pathogens.
作者简介
Anastasia Parakhina
Novosibirsk State Medical University
Email: an.parakhina@mail.ru
ORCID iD: 0000-0003-1108-0811
俄罗斯联邦, Novosibirsk
Larisa Parakhina
Novosibirsk State Medical University
Email: myinspirationmy@mail.ru
ORCID iD: 0000-0003-2428-9032
俄罗斯联邦, Novosibirsk
Darya Troshina
Center of Hygiene and Epidemiology in the Novosibirsk Region
Email: troshina.darya92@mail.ru
ORCID iD: 0000-0003-3658-2674
俄罗斯联邦, Novosibirsk
Katerina Levina
Center of Hygiene and Epidemiology in the Novosibirsk Region
Email: pumpum235@gmail.ru
ORCID iD: 0000-0001-6550-2931
俄罗斯联邦, Novosibirsk
Yulia Pimenova
Novosibirsk State Medical University
Email: medic.81@mail.ru
ORCID iD: 0000-0001-8722-2746
SPIN 代码: 6979-3750
MD, Cand. Sci. (Med.), Associate Professor
俄罗斯联邦, NovosibirskElena Koteneva
Novosibirsk State Medical University
Email: lenakoteneva@rambler.ru
ORCID iD: 0000-0002-9901-2292
SPIN 代码: 6638-0005
MD
俄罗斯联邦, NovosibirskAleksandr Evstropov
Novosibirsk State Medical University
Email: evstrop@gmail.com
ORCID iD: 0000-0002-1551-3154
SPIN 代码: 1747-5483
MD, Dr. Sci. (Med.), Professor
俄罗斯联邦, NovosibirskLiudmila Zakharova
Novosibirsk State Medical University
Email: nisngma@ngs.ru
ORCID iD: 0000-0001-6051-2380
SPIN 代码: 8610-8837
MD, Cand. Sci. (Med.), Associate Professor
俄罗斯联邦, NovosibirskAndrey Mironov
Moscow Research Institute of Epidemiology and Microbiology named after G.N. Gabrichevsky; Academy of Postgraduate Education of the Federal Scientific and Clinical Center for Specialized Types of Medical Care and Medical Technologies
Email: andy.60@mail.ru
ORCID iD: 0000-0002-8544-5230
SPIN 代码: 9225-1560
MD, Dr. Sci. (Med.), Professor
俄罗斯联邦, Moscow; MoscowTatyana Suranova
Academy of Postgraduate Education of the Federal Scientific and Clinical Center for Specialized Types of Medical Care and Medical Technologies
编辑信件的主要联系方式.
Email: suranovatatiana@mail.ru
ORCID iD: 0000-0003-3411-1027
SPIN 代码: 7326-5273
MD, Cand. Sci. (Med.), Associate Professor
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