Change in Functional Status of Native and Postthaw Bovine Sperm under Effects of Caffeine

Abstract

The effect of caffeine on the functional status of native and postthaw bovine sperm has been studied. Incubation of intact, nonfrozen bovine sperm with caffeine for 4 h resulted in increasing the number of capacitated and acrosome-reacted cells, while the combined use of prolactin and guanosine triphosphate had no effect on the correlation between capacitated, uncapacitated, and acrosome-reacted cells. Sperm preincubation and subsequent sperm freezing caused both a decrease in the capacitated cells and an increase in the acrosome-reacted cells in the thawed sperm samples in cases of adding caffeine, while they had no effect on the sperm’s functional status in cases when prolactin and guanosine triphosphate were used. Therefore, the sperm’s preincubation with caffeine before sperm freezing altered the functional status of cells after their thawing (decreasing the number of the capacitated cells), while the combined use of prolactin and guanosine triphosphate had no impact on this parameter.