Vital Stain in Milk-Based Medium to Evaluate Sperm Quality

Abstract

Assisted reproductive technology plays a key role in bovine reproduction in herds. Different media, including the milk-based medium, are used for bull semen cryopreservation. The objective of the survey was to develop a method for assessing sperm motility in frozen-thawed semen of the bull sires with the use of the vital stains added to the milk-based medium. It has been proven that vital staining with the use of Hoechst 33342 Dye in the milk-based medium is the most efficient in order to assess the motility of the frozen-thawed semen collected from the bull sires. The current method for semen detection can yield 100% accurate results.