Identification of agrobacteria on grape plants with symptoms of crown gall lesions in ampelocenosises of Krasnodar Territory using the PCR method

Abstract

It was shown that crown gall is a chronic disease of the grapevine caused by agrobacteria. The disease causes significant damage to the viticulture industry in Krasnodar Territory. The causative agent of crown gall in the vineyards of this region was identified and studied using the polymerase chain reaction method. Young tumor formations found on grape plants in different areas of Krasnodar Territory were analyzed. The presence of agrobacteria of species Agrobacterium vitis differing in the type of Ti-plasmid (oktopine, nopaline, or vitopine) were detected by using test-systems for Agrobacterium-mediated genes (pehA, virF, and virD2).