Determination of EC50 of water liozol of turpentine oil for acid eryhtrogram

Abstract

The cytotoxic in vitro effect of water turpentine oil liozols, intended as adjuvants for a number of vaccines, is investigated by the acid erythrogram method. It is found out that the acid resistance of erythrocytes does not change at a turpentine oil concentration below 0.0013 g/L in liozol and increases at a concentration of 0.0013–0.0125 g/L. If the concentration continues to increase, however, the acid resistance of erythrocytes decreases to reach the value of EC50 at a concentration of 0.1–0.125 g/L; then follows the zone of direct hemolytic action of liozol.