Specificity and Selectivity of a Modified Radioligand Method for Estimating the Binding Activity of β1 Adrenergic Receptors in Human T Lymphocytes

详细

A radioligand-based method was proposed for quantifying the binding activity of β1-adrenergic receptors (ARs) on the surface of human T lymphocytes. Using the transfected HEK293 cell lines expressing β1-AR (ADL-7A) and β2-AR (A2R9) as a model, the conditions for [¹²⁵I]cyanopindolol reliable detection of specific ligand binding to β1-adrenergic receptors at the level of 1–1.5 fmol per 1 million cells were selected. The real possibility of performing this analysis for clinical studies was shown by the example of human T lymphocytes.