Email this article Effect of Nanosecond RF Pulses on Mitochondrial Membranes
- Authors: Zharkova L.P.1,2, Romanchenko I.V.1, Bol’shakov M.A.1,2, Rostov V.V.1
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Affiliations:
- Institute of High-Current Electronics of the Siberian Branch of the Russian Academy of Sciences
- National Research Tomsk State University
- Issue: Vol 60, No 8 (2017)
- Pages: 1314-1318
- Section: Article
- URL: https://bakhtiniada.ru/1064-8887/article/view/238563
- DOI: https://doi.org/10.1007/s11182-017-1214-4
- ID: 238563
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Abstract
Effect of nanosecond RF pulses on the state of isolated mitochondria and their membranes is investigated. Mitochondrial suspensions are exposed to periodic RF pulses with durations from 4 to 25 ns, frequencies from 0.6 to 1.0 GHz, amplitudes from 0.1 to 36 kV/cm, and pulse repetition frequencies 8–25 Hz. The integrity of the mitochondrial membranes is estimated from their resistance to electric current. The possibility of opening of protein pores with nonspecific permeability is determined from a change in the mitochondrial volume by registration of optical density of organelle suspension.
About the authors
L. P. Zharkova
Institute of High-Current Electronics of the Siberian Branch of the Russian Academy of Sciences; National Research Tomsk State University
Author for correspondence.
Email: Zharkova_lubov@mail.ru
Russian Federation, Tomsk; Tomsk
I. V. Romanchenko
Institute of High-Current Electronics of the Siberian Branch of the Russian Academy of Sciences
Email: Zharkova_lubov@mail.ru
Russian Federation, Tomsk
M. A. Bol’shakov
Institute of High-Current Electronics of the Siberian Branch of the Russian Academy of Sciences; National Research Tomsk State University
Email: Zharkova_lubov@mail.ru
Russian Federation, Tomsk; Tomsk
V. V. Rostov
Institute of High-Current Electronics of the Siberian Branch of the Russian Academy of Sciences
Email: Zharkova_lubov@mail.ru
Russian Federation, Tomsk
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