Adsorption of Proteins on Silicalite-1 Surface Lipid Monolayers

Resumo

The adsorption of bovine serum albumin and bovine hemoglobin on silicalite-1 and monolayers of lecithin and cholesterol on a silicalite surface is investigated. Adsorption isotherms are obtained, and limit values of adsorption are determined that are 90, 75, and 60 mg/g for BSA and 35, 27, and 25 mg/g for hemoglobin on samples of silicalite, cholesterol/silicalite, and lecithin/silicalite, respectively. In the lecithin/silicalite system, proteins are adsorbed on both the lipid and silicalite. In the cholesterol/silicalite system, proteins are adsorbed only on the lipid monolayer. According to IR spectroscopy data, adsorption increases the part of disordered fragments in the secondary structure of protein.