Thermodynamic parameters analysis of ethidium bromide and mitoxantrone binding with DNA by adsorption isotherms

Resumo

Analysis of the adsorption isotherms by virtue of experimentally obtained data of anti-tumorous compound mitoxantrone and ethidium bromide binding with DNA has been carried out. The obtained data showed that throughout the comparatively simple linear isotherm, the more precise values of the binding constant K and number of nucleotides n, per binding site were received. Based on the values of K, those for the enthalpy changes at complex-formation of these ligands with DNA were obtained.