Том 71, № 5-6 (2016)

Methods of statistical data analysis for umbrella sampling simulations of enzyme catalytic reactions were considered using guanosine triphosphate hydrolysis by the Ras-GAP protein complex as an example.

One way to increase bioavailability and efficiency of drug substances is to decrease their particles up to nanoscale level and to change their crystal structure. A new stable nanoscale form of a polymorphic antibacterial 2,3-bis-(hydroxymethyl)-quinoxaline-N,N′-dioxyde (dioxidine) modification characterized with a gas chromatography, NMR, XRF, TEM, and thermoanalytic methods (TG, DTG, DSC) was obtained via cryochemical synthesis. The new polymorphic dioxidine modification was proved to be more active in growth inhibition processes of gram-positive M. cyaneum 98 and gram-negative E. coli bacterial strains than officinal modification.

Peritoneal dissemination and growth of tumor cells in the ascitic fluid in stages III and IV of recurrent ovarian cancer is difficult to treat; resistance to many anticancer drugs used in the treatment of solid ovarian cancer is observed at this stage. The author’s hypothesis that the differences in the molecular phenotype of tumor cells for various types of the disease could be an explanation for this. A comparative evaluation of the expression and coexpression of a number of molecular markers (immunofluorescence assay with flow cytometry) was performed using solid and ascitic ovarian cancer cells. In contrast to solid ovarian cancer, ascitic ovarian cancer cells present leukocyte common antigen CD45 and mesenchymal marker vimentin in addition to epithelial marker cytokeratin. In addition to the inhibition of anoikis (specific mechanism of epithelial cell death in a liquid medium in the absence of contact with the substrate), ascitic ovarian cancer cells are characterized by (1) emperipolesis (intracellular migration of leukocytes without damage to the tumor cell), and (2) phenotype of epithelial-mesenchymal transition. Thus, the data on clinically significant molecular differences between solid and recurrent ascitic ovarian cancer was first obtained, opening up opportunities for anticancer therapy, which previously was not used in the treatment of ovarian cancer.

PCR and ELISA methods for the detection of bovine leucosis in dried blood spots on porous membranes were compared. Dry samples were analyzed through real-time PCR, using several diagnostic test systems. Nineteen and 20 samples were identified as positive by PCR and ELISA, respectively. Fourteen of these samples were identified as positive by both methods. When using PCR and ELISA, 26 samples were identified as positive for leucosis, which amounted to 47% of the total number of tested samples. The results of the analysis of dried and native samples were in good agreement. The obtained results showed that whole blood sampling in the form of dried spots applied on membrane can be used as a convenient and reliable way to obtain dry samples of biological fluids with the purpose of screening herds for infectious diseases, in particular for bovine leucosis.

A composition of a kerogen was studied with the use of chemical, physical, and spectral (IR, NMR, XRD, XPS, and ESR) methods. A yield and main characteristics of kerogen were found.