Vol 71, No 3 (2016)

A method for the preparation of calcium phosphate particles is optimized. Particles with entrapped superoxide dismutase are obtained. The size, surface charge, and stability of the calcium phosphate particles are determined under different conditions. The kinetics of the enzyme release from the particles and the influence of the drug release on the size and surface charge of the particles are investigated. On the rabbit model of immunogenic uveitis (the model of an inflammatory process in the eye accompanied by oxidative stress), it is shown that superoxide dismutase enclosed in calcium phosphate particles has a higher antiinflammatory effect than superoxide dismutase in an aqueous solution.

The possibility of using glycol chitosan to obtain stabilized liposome containers for doxorubicin delivery is demonstrated. The dissociation constants of the liposome complexes with glycol chitosan (3.4 × 10^–4 and 1.10 × 10^–5 depending on the aggregation state of the liposomes) are determined. It is shown that the formation of liposome complexes with glycol chitosan has a significant prolongation effect on the release of doxorubicin from the liposomes at pH 7.4.

Sorption of some rare earth elements on filters with immobilized diethylenetriaminetetraacetate (DETATA) groups was studied. It was shown that the elements are quantitatively extracted from solutions with volume of 20–500 mL at pH values ranging between 3.5 and 5.5 and a flow rate from 1 to 8 mL/min. It was proposed to use masking agents such as sulfosalicylic acid and 1,10-phenanthroline for extracting elements from iron-containing solutions. The elements were determined directly on filters by X-ray fluorescence spectroscopy. The calibration dependences were shown to be linear in the concentration range from 2 to 100 μg of rare earth element per filter. The limits of detection of La, Ce, Pr, and Nd calculated on the basis of the 3s criterion in the case of extraction from 100 mL solutions were 0.03, 0.03, 0.01 and 0.01 μg/mL, respectively.

A modified method for the isolation of radionuclide ¹⁷⁷Lu obtained from a Yb-176 target irradiated with thermal neutrons is proposed. The method consists of the combination of two electrochemical processes— cementation of ytterbium acetate–chloride solution to sodium amalgam and subsequent electrolysis of the ytterbium solution in a separate electrolytic cell without adjusting the solution. The electrochemical setup consisting of two cells gives the purification factor of ¹⁷⁷Lu from ytterbium at a level of 10⁵–10⁶, which allows using ¹⁷⁷Lu of such quality for labeling bioorganic molecules.

Interfacial tensiometry of various liquids is an informative colloid-chemical method for the analysis of various liquids in many areas of natural sciences and medical practice. This method of dynamic surface tension (DST) measurements is proposed for the study of animal blood plasma or serum and mixtures of proteins, lipids, and salts as model systems. It is found that the saline affects lecithin aqueous dispersions significantly more weakly than those of the proteins. The DST values and biochemical parameters of the bovine blood serum of the same sample are obtained. Correlations between DST values and biochemical parameters of blood serum are found. They are both of fundamental and practical importance in laboratory diagnostics.