Transcriptomic Analysis Reveals Reduced Inorganic Sulfur Compound Oxidation Mechanism in Acidithiobacillus ferriphilus
- Авторы: Fan W.1, Peng Y.1, Meng Y.1, Zhang W.1, Zhu N.2, Wang J.1, Guo C.2, Li J.1, Du H.1, Dang Z.2
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Учреждения:
- Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering
- School of Environment and Energy
- Выпуск: Том 87, № 4 (2018)
- Страницы: 486-501
- Раздел: Experimental Articles
- URL: https://bakhtiniada.ru/0026-2617/article/view/163567
- DOI: https://doi.org/10.1134/S0026261718040070
- ID: 163567
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Аннотация
A new Acidithiobacillus sp. strain Acidithiobacillus ferriphilus (A. ferriphilus) SCUT-1 was identified, which belongs to the group IV Fe(II)-oxidizing acidithiobacilli. The genome of A. ferriphilus SCUT-1 was sequenced and assembled, and the genes related to sulfur metabolism were identified. Gene expression profiles of the strain cultured in Fe2+, S0, and FeS2 medium were compared (FeS2/Fe2+, S0/Fe2+, and FeS2/S0) in order to identify the genes involved in RISC (reduced inorganic sulfur compound) and ferrous iron (Fe2+) oxidation in A. ferriphilus SCUT-1, with the method of transcriptome reconstruction based on RNA-Seq and genome-guided transcriptome assembly. The soxY-Z-B genes encoding sulfur oxidation proteins, the soe gene cluster encoding sulfite oxidation complex, the hdr gene cluster involved in encoding HDR complex and sulfur transfer proteins, and the cyoA-B-C-D gene cluster encoding bo3 oxidase were were significantly up-regulated in sulfur-containing media, suggesting their engagement in reduced sulfur oxidation in A. ferriphilus SCUT-1. Based on the differentially expressed genes and the putative genes predicted from the genome sequence, the RISC oxidation model and the genes involved in ferrous iron oxidation in A. ferriphilus SCUT-1 were preliminarily elucidated, which helps to interpret the oxidation mechanism of RISC and Fe2+ in acidithiobacilli.
Об авторах
W. Fan
Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering
Email: hldu@scut.edu.cn
Китай, Guangzhou, 510006
Y. Peng
Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering
Email: hldu@scut.edu.cn
Китай, Guangzhou, 510006
Y. Meng
Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering
Email: hldu@scut.edu.cn
Китай, Guangzhou, 510006
W. Zhang
Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering
Email: hldu@scut.edu.cn
Китай, Guangzhou, 510006
N. Zhu
School of Environment and Energy
Email: hldu@scut.edu.cn
Китай, Guangzhou, 510006
J. Wang
Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering
Email: hldu@scut.edu.cn
Китай, Guangzhou, 510006
Ch. Guo
School of Environment and Energy
Email: hldu@scut.edu.cn
Китай, Guangzhou, 510006
J. Li
Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering
Email: hldu@scut.edu.cn
Китай, Guangzhou, 510006
H. Du
Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering
Автор, ответственный за переписку.
Email: hldu@scut.edu.cn
Китай, Guangzhou, 510006
Z. Dang
School of Environment and Energy
Email: hldu@scut.edu.cn
Китай, Guangzhou, 510006
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