Salicola mahdashtensis sp. nov., an extremely halophilic bacterium isolated from Mahdasht saline spring in Iran


Citar

Texto integral

Acesso aberto Acesso aberto
Acesso é fechado Acesso está concedido
Acesso é fechado Somente assinantes

Resumo

A novel extremely halophilic bacterium, designated strain R1T, was isolated from saline spring bed soil collected from Mahdasht in Alborz province, Iran. Strain R1T is gram negative, motile, reddish orange pigmented, rod shape, does not form endospores, facultative anaerobe required at least 17.5% salt and 20% total salinity for growth. Optimal growth was occurred in 20% salt and growth observed in salt more than 30%. pH ranges between 5.5 to 8.5, optimum pH for growth is 6.5. Cellular fatty acids are C10:0, C12:0, C12:03-OH, C16:0 Nalcohol, C16:0, C18:3ω6c (6, 9, 12) and C18:1ω9c. Phylogenetic analysis of 16S rRNA gene sequence showed a close proximity to Salicola salis (99.2%) and Salicola marasensis (99.0%) in the Gammaproteobacteria. The G + C content of type strain was 61.3 mol %. DNA−DNA hybridization indicated that the level of relatedness to Salicola salis was 65.1% and that to Salicola marasensis was 63.5%. Further differences were apparent in antibiotic resistance, oxidase activity, nitrate reduction, hydrolysis of pectin and growth on citrate medium, utilize glucose and lactose. Based on the phenotypic and genotypic data presented, strain R1T should be the type strain of a new species of genus Salicola which has been named Salicola mahdashtensis. The type strain is R1T (KCTC 32441, MTCC 11814).

Sobre autores

M. Al-Rubaye

Department of Microbiology, Faculty of Biological Sciences

Email: maryamhosseini83@gmail.com
Irã, Tehran

J. Fakhari

Department of Microbiology, Faculty of Biological Sciences

Email: maryamhosseini83@gmail.com
Irã, Tehran

M. Hosseini

Department of Microbiology, Faculty of Biological Sciences

Autor responsável pela correspondência
Email: maryamhosseini83@gmail.com
Irã, Tehran

A. Bakhtiari

Department of Microbiology, Faculty of Sciences, Karaj Branch

Email: maryamhosseini83@gmail.com
Irã, Alborz

Arquivos suplementares

Arquivos suplementares
Ação
1. JATS XML

Declaração de direitos autorais © Pleiades Publishing, Ltd., 2017