Salicola mahdashtensis sp. nov., an extremely halophilic bacterium isolated from Mahdasht saline spring in Iran
- Autores: Al-Rubaye M.T.1, Fakhari J.1, Hosseini M.1, Bakhtiari A.2
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Afiliações:
- Department of Microbiology, Faculty of Biological Sciences
- Department of Microbiology, Faculty of Sciences, Karaj Branch
- Edição: Volume 86, Nº 2 (2017)
- Páginas: 213-217
- Seção: Experimental Articles
- URL: https://bakhtiniada.ru/0026-2617/article/view/163016
- DOI: https://doi.org/10.1134/S0026261717020035
- ID: 163016
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Resumo
A novel extremely halophilic bacterium, designated strain R1T, was isolated from saline spring bed soil collected from Mahdasht in Alborz province, Iran. Strain R1T is gram negative, motile, reddish orange pigmented, rod shape, does not form endospores, facultative anaerobe required at least 17.5% salt and 20% total salinity for growth. Optimal growth was occurred in 20% salt and growth observed in salt more than 30%. pH ranges between 5.5 to 8.5, optimum pH for growth is 6.5. Cellular fatty acids are C10:0, C12:0, C12:03-OH, C16:0 Nalcohol, C16:0, C18:3ω6c (6, 9, 12) and C18:1ω9c. Phylogenetic analysis of 16S rRNA gene sequence showed a close proximity to Salicola salis (99.2%) and Salicola marasensis (99.0%) in the Gammaproteobacteria. The G + C content of type strain was 61.3 mol %. DNA−DNA hybridization indicated that the level of relatedness to Salicola salis was 65.1% and that to Salicola marasensis was 63.5%. Further differences were apparent in antibiotic resistance, oxidase activity, nitrate reduction, hydrolysis of pectin and growth on citrate medium, utilize glucose and lactose. Based on the phenotypic and genotypic data presented, strain R1T should be the type strain of a new species of genus Salicola which has been named Salicola mahdashtensis. The type strain is R1T (KCTC 32441, MTCC 11814).
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Sobre autores
M. Al-Rubaye
Department of Microbiology, Faculty of Biological Sciences
Email: maryamhosseini83@gmail.com
Irã, Tehran
J. Fakhari
Department of Microbiology, Faculty of Biological Sciences
Email: maryamhosseini83@gmail.com
Irã, Tehran
M. Hosseini
Department of Microbiology, Faculty of Biological Sciences
Autor responsável pela correspondência
Email: maryamhosseini83@gmail.com
Irã, Tehran
A. Bakhtiari
Department of Microbiology, Faculty of Sciences, Karaj Branch
Email: maryamhosseini83@gmail.com
Irã, Alborz
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