Production of antimicrobial polypeptides by Propionibacterium freudenreichii RVS-4-irf

Abstract

Propionibacterium freudenreichii RVS-4-irf is a probiotic bacterium producing antimicrobial exometabolites applicable for foodstuff protection. Production of antimicrobial factors other than low-molecular propionates was observed in media with and without trypton. A method was developed for production of the fraction of high-molecular mass exopolymers from the culture liquid. Their polypeptide nature was confirmed using proteinase K. The preparation of extracellular polypeptides from P. freudenreichii RVS-4-irf exhibited species-specific activity in suppression of fungal and bacterial growth.