Methacrylate-Reducing Activity of Anaerobic Bacteria Anaeromyxobacter dehalogenans and Denitrovibrio acetiphilus

Abstract

Periplasmic methacrylate-reducing activity was shown for anaerobic acetate-oxidizing gram-negative bacteria Anaeromyxobacter dehalogenans 2CP-1^Т (class Deltaproteobacteria) and Denitrovibrio acetiphilus DSM 12809^Т (class Deferribacteres), both possessing homologous genes for methacrylate redox system components. No acrylate reductase activity was revealed in these bacteria. A. dehalogenans and D. acetiphilus were also found to reduce fumarate, exhibiting periplasmic and intracellular fumarate reductase activity, respectively. D. acetiphilus was found to possess nitrate reductase activity of periplasmic and intracellular localization. The possible correspondence between reductase activities and the hypothetical proteins genes in the known genomes of A. dehalogenans and D. acetiphilus are discussed.