Quenchers Protect BChl850 from Action of Singlet Oxygen in the Membranes of a Sulfur Photosynthetic Bacterium Allochromatium vinosum Strain MSU

Abstract

The effect of singlet oxygen was studied in the system of Allochromatium (Alc.) vinosum MSU membranes, rose bengal, and light (547−600nm) with and without a quencher. In the system without a quencher, bleaching of the BChl850 band of the LH2 light-harvesting complex was observed and an absorption peak emerged at 698 nm, which belonged to the oxidized product, 3-acetyl-chlorophyll. The efficiency of five quenchers of singlet oxygen for neutralization of the effect of singlet oxygen on bacteriochlorophyll in the light-harvesting complexes of the membranes Alc. vinosum MSU was studied: sodium ascorbate, histidine, imidazole, trolox, and sodium azide. All of them, with the exception of sodium azide, neutralized successively the effect of singlet oxygen.