Effect of Constitutive Expression of the rapA1 Gene on Formation of Bacterial Biofilms and Growth-Stimulating Activity of Rhizobia


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Abstract

In order to investigate the effect of the RapA1 protein on growth-stimulating activity, biofilm formation, and root colonization by rhizobia, strains Rhizobium leguminosarum Pvu5, VSy12, Thy2, TPr4 and R. galegae 0702 were transformed with the pJN105TurboRapA1GFP vector, providing for constitutive expression of the rapA1 and gfp genes. Constitutive expression of the rapA1 gene was shown to result in more efficient biofilm formation on inert surfaces and to promote microcolony formation on plant root surfaces. Analysis of auxin production by wild and transformed strains revealed decreased auxin synthesis in all transformed strains, except for R. leguminosarum Thy2. Growth-stimulating activity of the wild and transformed strains did not correlate with the amount of auxins produced in vitro.

About the authors

Z. R. Vershinina

Institute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Author for correspondence.
Email: zilyaver@mail.ru
Russian Federation, Ufa, 450054

L. R. Khakimova

Institute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Email: baymiev@mail.ru
Russian Federation, Ufa, 450054

A. M. Lavina

Institute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Email: baymiev@mail.ru
Russian Federation, Ufa, 450054

L. R. Karimova

Institute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Email: baymiev@mail.ru
Russian Federation, Ufa, 450054

E. R. Serbaeva

Bashkir State University

Email: baymiev@mail.ru
Russian Federation, Ufa, 450076

V. I. Safronova

All-Russian Research Institute of Agricultural Microbiology

Email: baymiev@mail.ru
Russian Federation, St.-Petersburg, 196608

A. I. Shaposhnikov

All-Russian Research Institute of Agricultural Microbiology

Email: baymiev@mail.ru
Russian Federation, St.-Petersburg, 196608

An. Kh. Baimiev

Institute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Email: baymiev@mail.ru
Russian Federation, Ufa, 450054

Al. Kh. Baimiev

Institute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Author for correspondence.
Email: baymiev@mail.ru
Russian Federation, Ufa, 450054

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