Structural Peculiarities and Biological Properties of the Lipopolysaccharide from Herbaspirillum seropedicae Z78
- 作者: Velichko N.S.1, Surkina A.K.1, Fedonenko Y.P.1,2, Zdorovenko E.L.3, Konnova S.A.1,2
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隶属关系:
- Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences
- Chernyshevsky Saratov State University
- Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
- 期: 卷 87, 编号 5 (2018)
- 页面: 635-641
- 栏目: Experimental Articles
- URL: https://bakhtiniada.ru/0026-2617/article/view/163652
- DOI: https://doi.org/10.1134/S002626171805017X
- ID: 163652
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详细
Lipopolysaccharide was isolated by phenol extraction from the surface membrane of the nitrogen-fixing endophytic rhizobacterium Herbaspirillum seropedicae, strain Z78. The lipopolysaccharide’s lipid A contained 3-hydroxydecanoic, 3-hydroxydodecanoic, dodecanoic, tetradecanoic, and hexadecanoic acids. The 3-hydroxydodecanoic acid was amide-linked to the sugar backbone of the lipid A. The structure of the O-polysaccharide from H. seropedicae Z78 was established for the first time. It is characterized by heterogeneity and by the presence of glycerol, a component rarely found in gram-negative bacteria. The O polysaccharide of H. seropedicae Z78 was found to consist of two types of repeating units: one represented by glycerol-1-phosphate and the other by the glycerol-1-phosphate of the backbone, which is substituted at the 2-position by N-acetyl-D-glucosamine. The lipopolysaccharide of the H. seropedicae Z78 was weakly toxic to warm-blooded animals and moderately and dose-dependently induced interleukin synthesis by human whole blood cells and NO synthesis by mouse splenocytes. This may indicate that the H. seropedicae lipopolysaccharide is a promising antagonist of classical endotoxins.
作者简介
N. Velichko
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: velichko_n@ibppm.ru
俄罗斯联邦, Saratov
A. Surkina
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences
Email: velichko_n@ibppm.ru
俄罗斯联邦, Saratov
Y. Fedonenko
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences; Chernyshevsky Saratov State University
Email: velichko_n@ibppm.ru
俄罗斯联邦, Saratov; Saratov
E. Zdorovenko
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
Email: velichko_n@ibppm.ru
俄罗斯联邦, Moscow
S. Konnova
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences; Chernyshevsky Saratov State University
Email: velichko_n@ibppm.ru
俄罗斯联邦, Saratov; Saratov
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