Isolation of extremely halophilic Archaea from a saline river in the Lut Desert of Iran, moderately resistant to desiccation and gamma radiation
- 作者: Shirsalimian M.S.1, Amoozegar M.A.2, Sepahy A.A.1, Kalantar S.M.3, Dabbagh R.4
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隶属关系:
- Department of Microbiology, Faculty of Biological Sciences
- Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science
- Genetics Department
- Nuclear Sciences and Technology Research Institute
- 期: 卷 86, 编号 3 (2017)
- 页面: 403-411
- 栏目: Experimental Articles
- URL: https://bakhtiniada.ru/0026-2617/article/view/163129
- DOI: https://doi.org/10.1134/S0026261717030158
- ID: 163129
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详细
Admittedly, the Lut Desert of Iran has been remained as an unexplored region from a microbiological standpoint. Domain Archaea contains extremophiles that can live in harsh habitats. Extremely halophilic archaea are exposed to different environmental stresses in the hypersaline environments such as high solar irradiance and periodic desiccation. Haloarchaeal diversity in Shoor River, a saline river in the Lut Desert (a salinity of 134.3 g L–1 of dissolved salts), was investigated by a culture-dependent method. A large number of extremely halophilic isolates were obtained and a subset of 59 isolates was considered distinct. Firstly, the isolates were screened for their resistance under desiccation stress in 35 days. Eleven of these strains remained viable during the period in a desiccator containing silica gel. Then, three of them were randomly selected and their resistance against desiccation and ionizing radiation were determined. The isolates MS2, MS17, and MS50 were still recovered after 8 weeks in a desiccator and were moderately resistant to gamma radiation with D10 value between 2 and 3 kGy. Strains MS2, MS17, and MS50 were affiliated with three species in the family Halobacteriaceae using 16S rRNA gene sequence analysis as well as morphological and biochemical characteristics—Haloterrigena jeotgali A29T (99.6% similarity), Natrialba aegyptia 40T (99.4% similarity) and Natrinema pallidum NCIMB 777T (99.3% similarity), respectively. Although resistance to desiccation did not follow the sigmoid survival curve pattern of Deinococcus radiodurans, apparently haloarchaea can show a more resistance to desiccation in more long-term periods of time. This is the first report on isolation of extremely halophilic archaea belonged to the family Halobacteriaceae and their radioresistance and desiccation tolerance properties isolated from the Shoor River.
作者简介
M. Shirsalimian
Department of Microbiology, Faculty of Biological Sciences
Email: amozegar@khayam.ut.ac.ir
伊朗伊斯兰共和国, Tehran
M. Amoozegar
Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science
编辑信件的主要联系方式.
Email: amozegar@khayam.ut.ac.ir
伊朗伊斯兰共和国, Tehran
A. Sepahy
Department of Microbiology, Faculty of Biological Sciences
Email: amozegar@khayam.ut.ac.ir
伊朗伊斯兰共和国, Tehran
S. Kalantar
Genetics Department
Email: amozegar@khayam.ut.ac.ir
伊朗伊斯兰共和国, Yazd
R. Dabbagh
Nuclear Sciences and Technology Research Institute
Email: amozegar@khayam.ut.ac.ir
伊朗伊斯兰共和国, Tehran
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