Sporulation of Bacillus subtilis in binary cultures with ultramicrobacteria


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Abstract

The effect of ultramicrobacterial epibionts of the genera Kaistia (strain NF1), Chryseobacterium (strain NF4), and Stenotrophomonas (strain FM3) on the process of sporulation of Bacillus subtilis ATCC 6633 was studied. The investigated strains of ultramicrobacteria (UMB) were found to inhibit the sporulation process of B. subtilis ATCC 6633 in binary mixed cultures, exhibiting a 3-day delay of the onset of sporulation compared to the control one, an extended period of the prospore maturation, formation of the fraction of immature spores, and development of ultrastructural defects in many endospores. Thus, investigation of binary mixed cultures of B. subtilis and UMB revealed that, apart from suppression of reproduction and lysis of host vegetative cells, inhibition of spore formation and destruction of endospores was yet another feature of intermicrobial parasitism. The UMB parasites of the studied genera are assumed to participate in the regulation of development and reproduction of B. subtilis in natural habitats of this spore-forming bacterium.

About the authors

A. P. Shorokhova

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Author for correspondence.
Email: shorann@rambler.ru
Russian Federation, Pushchino

N. E. Suzina

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: shorann@rambler.ru
Russian Federation, Pushchino

V. N. Polivtseva

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: shorann@rambler.ru
Russian Federation, Pushchino

T. Z. Esikova

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: shorann@rambler.ru
Russian Federation, Pushchino

D. V. Ross

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: shorann@rambler.ru
Russian Federation, Pushchino

V. P. Kholodenko

Pushchino State University of Natural Sciences

Email: shorann@rambler.ru
Russian Federation, Pushchino

T. N. Abashina

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: shorann@rambler.ru
Russian Federation, Pushchino

V. I. Duda

Skryabin Institute of Biochemistry and Physiology of Microorganisms; Pushchino State University of Natural Sciences

Email: shorann@rambler.ru
Russian Federation, Pushchino; Pushchino

A. M. Boronin

Skryabin Institute of Biochemistry and Physiology of Microorganisms; Pushchino State University of Natural Sciences

Email: shorann@rambler.ru
Russian Federation, Pushchino; Pushchino

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