Role of the microbial community in formation of speleothem (moonmilk) in the Snezhnaya carst cave (abkhazia)
- Authors: Kondratyeva L.M.1, Polevskaya O.S.1, Litvinenko Z.N.1, Golubeva E.M.2, Konovalova N.S.2
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Affiliations:
- Institute of Water and Ecological Problems, Far Eastern Branch
- Yu. A. Kosygin Institute of Tectonics and Geophysics, Far Eastern Branch
- Issue: Vol 85, No 5 (2016)
- Pages: 629-637
- Section: Experimental Articles
- URL: https://bakhtiniada.ru/0026-2617/article/view/162840
- DOI: https://doi.org/10.1134/S002626171605009X
- ID: 162840
Cite item
Abstract
The results of investigation of speleothem (moonmilk) from the Snezhnaya cave (West Caucasus, Abkhazia) are reported. The structure of microbial complexes from moonmilk was investigated by quantitative PCR; strains of culturable microorganisms were isolated and their preferred temperature and carbon sources were determined. Among eubacteria, ~34% belonged to the iron bacteria (Rhodoferax). Most bacterial strains were shown to be facultative psychrophiles with the maximum growth rate at 4°C. The microstructure and elemental composition of moonmilk were investigated using a scanning electron microscope (EVO-40HV, Carl Zeiss) and silicon drift X-ray detector X-MAX 80mm2. The visually plastic and homogeneous mass of moonmilk was shown to be highly heterogeneous, containing various microstructures. The elemental composition of some nanostructures depended on the structure of bacterial biofilms. Some loci of the biofilm were found to contain up to 46% (wt/wt) of iron oxides. High calcium content, up to 61.5% (wt/wt), was found only in cubic crystalline structures which were not involved in microbiological processes.
About the authors
L. M. Kondratyeva
Institute of Water and Ecological Problems, Far Eastern Branch
Email: zoyana2003@mail.ru
Russian Federation, Khabarovsk
O. S. Polevskaya
Institute of Water and Ecological Problems, Far Eastern Branch
Email: zoyana2003@mail.ru
Russian Federation, Khabarovsk
Z. N. Litvinenko
Institute of Water and Ecological Problems, Far Eastern Branch
Author for correspondence.
Email: zoyana2003@mail.ru
Russian Federation, Khabarovsk
E. M. Golubeva
Yu. A. Kosygin Institute of Tectonics and Geophysics, Far Eastern Branch
Email: zoyana2003@mail.ru
Russian Federation, Khabarovsk
N. S. Konovalova
Yu. A. Kosygin Institute of Tectonics and Geophysics, Far Eastern Branch
Email: zoyana2003@mail.ru
Russian Federation, Khabarovsk
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