Role of the microbial community in formation of speleothem (moonmilk) in the Snezhnaya carst cave (abkhazia)


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

The results of investigation of speleothem (moonmilk) from the Snezhnaya cave (West Caucasus, Abkhazia) are reported. The structure of microbial complexes from moonmilk was investigated by quantitative PCR; strains of culturable microorganisms were isolated and their preferred temperature and carbon sources were determined. Among eubacteria, ~34% belonged to the iron bacteria (Rhodoferax). Most bacterial strains were shown to be facultative psychrophiles with the maximum growth rate at 4°C. The microstructure and elemental composition of moonmilk were investigated using a scanning electron microscope (EVO-40HV, Carl Zeiss) and silicon drift X-ray detector X-MAX 80mm2. The visually plastic and homogeneous mass of moonmilk was shown to be highly heterogeneous, containing various microstructures. The elemental composition of some nanostructures depended on the structure of bacterial biofilms. Some loci of the biofilm were found to contain up to 46% (wt/wt) of iron oxides. High calcium content, up to 61.5% (wt/wt), was found only in cubic crystalline structures which were not involved in microbiological processes.

About the authors

L. M. Kondratyeva

Institute of Water and Ecological Problems, Far Eastern Branch

Email: zoyana2003@mail.ru
Russian Federation, Khabarovsk

O. S. Polevskaya

Institute of Water and Ecological Problems, Far Eastern Branch

Email: zoyana2003@mail.ru
Russian Federation, Khabarovsk

Z. N. Litvinenko

Institute of Water and Ecological Problems, Far Eastern Branch

Author for correspondence.
Email: zoyana2003@mail.ru
Russian Federation, Khabarovsk

E. M. Golubeva

Yu. A. Kosygin Institute of Tectonics and Geophysics, Far Eastern Branch

Email: zoyana2003@mail.ru
Russian Federation, Khabarovsk

N. S. Konovalova

Yu. A. Kosygin Institute of Tectonics and Geophysics, Far Eastern Branch

Email: zoyana2003@mail.ru
Russian Federation, Khabarovsk

Supplementary files

Supplementary Files
Action
1. JATS XML

Copyright (c) 2016 Pleiades Publishing, Ltd.