Phylogenetic and functional prokaryotic diversity in the Hoito-Gol mesothermal mineral spring (Eastern Sayan Mountains, Buryat Republic)
- Authors: Kashkak E.S.1, Bel’kova N.L.2, Danilova E.V.3, Dagurova O.P.3, Namsaraev B.B.3, Gorlenko V.M.4
-
Affiliations:
- Baikal Institute of Nature Management, Siberian Branch
- Limnological Institute, Siberian Branch
- Institute of General and Experimental Biology, Siberian Branch
- Winogradsky Institute of Microbiology, Research Center of Biotechnology
- Issue: Vol 85, No 5 (2016)
- Pages: 592-603
- Section: Experimental Articles
- URL: https://bakhtiniada.ru/0026-2617/article/view/162810
- DOI: https://doi.org/10.1134/S0026261716050076
- ID: 162810
Cite item
Abstract
High-throughput sequencing was used for comparative analysis of microbial communities of the water and mat from the Hoito-Gol mesothermal mineral sulfide spring (Eastern Sayan Mountains, Buryat Republic). Activity of microbial communities was determined. While both spring biotopes were dominated by members of three bacterial phyla—Proteobacteria, Bacteroidetes, and Firmicutes—they differed drastically in the composition of predominant phylotypes (at the genus level). In the water, the organisms widespread in aquatic environments were predominant, mostly aerobic chemoorganotrophs of the genera Acinetobacter, Pedobacter, and Flavobacterium. In the microbial mat, the organisms actively involved in the sulfur cycle predominated, including sulfur-reducing bacteria Sulfurospirillum, sulfate-reducing deltaproteobacteria, sulfuroxidizing chemoautotrophic bacteria, anoxygenic phototrophic bacteria of the phyla Chloroflexi and Chlorobi, as well as purple bacteria belonging to the α-, ß-, and γ-Proteobacteria. Microbial mats of the spring exhibited higher phylogenetic diversity compared to high-temperature mats containing photosynthetic microorganisms.
About the authors
E. S. Kashkak
Baikal Institute of Nature Management, Siberian Branch
Author for correspondence.
Email: klslena@yandex.ru
Russian Federation, Ulan-Ude
N. L. Bel’kova
Limnological Institute, Siberian Branch
Email: klslena@yandex.ru
Russian Federation, Irkutsk
E. V. Danilova
Institute of General and Experimental Biology, Siberian Branch
Email: klslena@yandex.ru
Russian Federation, Ulan-Ude
O. P. Dagurova
Institute of General and Experimental Biology, Siberian Branch
Email: klslena@yandex.ru
Russian Federation, Ulan-Ude
B. B. Namsaraev
Institute of General and Experimental Biology, Siberian Branch
Email: klslena@yandex.ru
Russian Federation, Ulan-Ude
V. M. Gorlenko
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: klslena@yandex.ru
Russian Federation, Moscow
Supplementary files
