Hydrogenogenic and sulfidogenic growth of Thermococcus archaea on carbon monoxide and formate
- Authors: Kozhevnikova D.A.1, Taranov E.A.1, Lebedinsky A.V.1, Bonch-Osmolovskaya E.A.1, Sokolova T.G.1
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Affiliations:
- Winogradsky Institute of Microbiology, Research Center of Biotechnology
- Issue: Vol 85, No 4 (2016)
- Pages: 400-410
- Section: Experimental Articles
- URL: https://bakhtiniada.ru/0026-2617/article/view/162686
- DOI: https://doi.org/10.1134/S0026261716040135
- ID: 162686
Cite item
Abstract
Enrichment and pure cultures of hyperthermophilic archaea capable of anaerobic growth on one-carbon compounds (CO and/or formate) were obtained from deep-sea sites of hydrothermal activity at the Mid-Atlantic Ridge, Lau Basin, and Guaymas Basin. All isolates belonged to the T. barophilus‒T. paralvinellae group within the genus Thermococcus. In all cases available for analysis, the genomes of Thermococcus strains capable of growth by hydrogenogenic utilization of CO and/or formate contained clusters of genes encoding energy-converting hydrogenase and either CO dehydrogenase or formate dehydrogenase and formate transporter. Apart from the previously known processes of hydrogenogenic oxidation of CO and formate, the oxidation of these substrates coupled to sulfur reduction was observed, processes previously unknown among archaea. The capacities for hydrogenogenic or sulfidogenic oxidation of CO and formate occurred in the studied strains in all possible combinations, which could only in part be explained by peculiarities of organization of genetic determinants revealed in the genomes. Investigation of CO and formate consumption kinetics revealed that T. barophilus strain Ch5 was able to grow at concentrations close to the environmental ones. Thus, it was shown that hyperthermophilic archaea from deep-sea hydrothermal vents are able to utilize one-carbon substrates of abiotic origin both in the presence of an electron acceptor (sulfur) and in its absence. These processes were probably of importance under the conditions of the early Earth biosphere.
About the authors
D. A. Kozhevnikova
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: tatso2204@gmail.com
Russian Federation, Moscow
E. A. Taranov
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: tatso2204@gmail.com
Russian Federation, Moscow
A. V. Lebedinsky
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: tatso2204@gmail.com
Russian Federation, Moscow
E. A. Bonch-Osmolovskaya
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: tatso2204@gmail.com
Russian Federation, Moscow
T. G. Sokolova
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Author for correspondence.
Email: tatso2204@gmail.com
Russian Federation, Moscow
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