Metagenomic analysis of microbial communities of the sediments of the Kara Sea shelf and the Yenisei Bay


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Abstract

Microbial diversity in the sediments of the Kara Sea shelf and the southern Yenisei Bay, differing in pore water mineralization, was studied using massive parallel pyrosequencing according to the 454 (Roche) technology. Members of the same phyla (Cyanobacteria, Verrucomicrobia, Actinobacteria, Proteobacteria, and Bacteroidetes) predominated in bacterial communities of the sediments, while their ratio and taxonomic composition varied within the phyla and depended on pore water mineralization. Increasing salinity gradient was found to coincide with increased share of the γ-Proteobacteria and decreased abundance of α- and β-Proteobacteria, as well as of the phyla Verrucomicrobia, Chloroflexi, Chlorobi, and Acidobacteria. Archaeal diversity was lower, with Thaumarchaeota predominant in the sediments with high and low mineralization, while Crenarchaeota predominated in moderately mineralized sediments. Microbial communities of the Kara Sea shelf and Yenisei Bay sediments were found to contain the organisms capable of utilization of a broad spectrum of carbon sources, including gaseous and petroleum hydrocarbons.

About the authors

E. V. Mamaeva

Limnological Institute, Siberian Branch

Author for correspondence.
Email: elena_m@lin.irk.ru
Russian Federation, Irkutsk

Yu. P. Galach’yants

Limnological Institute, Siberian Branch

Email: elena_m@lin.irk.ru
Russian Federation, Irkutsk

K. V. Khabudaev

Limnological Institute, Siberian Branch

Email: elena_m@lin.irk.ru
Russian Federation, Irkutsk

D. P. Petrova

Limnological Institute, Siberian Branch

Email: elena_m@lin.irk.ru
Russian Federation, Irkutsk

T. V. Pogodaeva

Limnological Institute, Siberian Branch

Email: elena_m@lin.irk.ru
Russian Federation, Irkutsk

T. B. Khodzher

Limnological Institute, Siberian Branch

Email: elena_m@lin.irk.ru
Russian Federation, Irkutsk

T. I. Zemskaya

Limnological Institute, Siberian Branch

Email: elena_m@lin.irk.ru
Russian Federation, Irkutsk

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