Changes in cell surface properties and biofilm formation efficiency in Azospirillum brasilense Sp245 mutants in the putative genes of lipid metabolism mmsB1 and fabG1


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Abstract

The previously obtained insertion mutants of Azospirillum brasilense Sp245 in the genes mmsB1 and fabG1 (strains SK039 and Sp245.1610, respectively) were characterized by impaired flagellation and motility. The putative products of expression of these genes are 3-hydroxyisobutyrate dehydrogenase and 3-oxoacyl-[acyl-carrier protein] reductase, respectively. In the present work, A. brasilense strains Sp245, SK039, and Sp245.1610 were found to have differences in the content of 3-hydroxyhexadecanoic, hexadecanoic, 3-hydroxytetradecanoic, hexadecenoic, octadecenoic, and nonadecanoic acids in their lipopolysaccharide preparations, as well as in cell hydrophobicity and hemagglutination activity and dynamics of cell aggregation, in biomass amount, and in the relative content of lipopolysaccharide antigens in mature biofilms formed on hydrophilic or hydrophobic surfaces.

About the authors

E. M. Shumilova

Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: ei_katsy@mail.ru
Russian Federation, Saratov

A. V. Shelud’ko

Institute of Biochemistry and Physiology of Plants and Microorganisms

Author for correspondence.
Email: shel71@yandex.ru
Russian Federation, Saratov

Yu. A. Filip’echeva

Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: ei_katsy@mail.ru
Russian Federation, Saratov

S. S. Evstigneeva

Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: ei_katsy@mail.ru
Russian Federation, Saratov

E. G. Ponomareva

Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: ei_katsy@mail.ru
Russian Federation, Saratov

L. P. Petrova

Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: ei_katsy@mail.ru
Russian Federation, Saratov

E. I. Katsy

Institute of Biochemistry and Physiology of Plants and Microorganisms

Author for correspondence.
Email: ei_katsy@mail.ru
Russian Federation, Saratov

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