Characterization of the structure of the prokaryotic complex of Antarctic permafrost by molecular genetic techniques
- Authors: Manucharova N.A.1, Trosheva E.V.1, Kol’tsova E.M.1, Demkina E.V.2, Karaevskaya E.V.3, Rivkina E.M.3, Mardanov A.V.4, El’-Registan G.I.2
-
Affiliations:
- Department of Soil Biology, Faculty of Soil Science
- Winogradsky Institute of Microbiology, Research Center of Biotechnology
- Institute of Physicochemical and Biological Problems of Soil Science
- Institute of Bioengineering, Research Center of Biotechnology
- Issue: Vol 85, No 1 (2016)
- Pages: 102-108
- Section: Experimental Articles
- URL: https://bakhtiniada.ru/0026-2617/article/view/162486
- DOI: https://doi.org/10.1134/S0026261716010057
- ID: 162486
Cite item
Abstract
A prokaryotic mesophilic organotrophic community responsible for 10% of the total microbial number determined by epifluorescence microscopy was reactivated in the samples of Antarctic permafrost retrieved from the environment favoring long-term preservation of microbial communities (7500 years). No culturable forms were obtained without resuscitation procedures (CFU = 0). Proteobacteria, Actinobacteria, and Firmicutes were the dominant microbial groups in the complex. Initiation of the reactivated microbial complex by addition of chitin (0.1% wt/vol) resulted in an increased share of metabolically active biomass (up to 50%) due to the functional domination of chitinolytics caused by the target resource. Thus, sequential application of resuscitation procedures and initiation of a specific physiological group (in this case, chitinolytics) to a permafrost-preserved microbial community made it possible to reveal a prokaryotic complex capable of reversion of metabolic activity (FISH data), to determine its phylogenetic structure by metagenomic analysis, and to isolate a pure culture of the dominant microorganism with high chitinolytic activity.
About the authors
N. A. Manucharova
Department of Soil Biology, Faculty of Soil Science
Author for correspondence.
Email: manucharova@mail.ru
Russian Federation, Moscow
E. V. Trosheva
Department of Soil Biology, Faculty of Soil Science
Email: manucharova@mail.ru
Russian Federation, Moscow
E. M. Kol’tsova
Department of Soil Biology, Faculty of Soil Science
Email: manucharova@mail.ru
Russian Federation, Moscow
E. V. Demkina
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: manucharova@mail.ru
Russian Federation, Moscow
E. V. Karaevskaya
Institute of Physicochemical and Biological Problems of Soil Science
Email: manucharova@mail.ru
Russian Federation, Moscow
E. M. Rivkina
Institute of Physicochemical and Biological Problems of Soil Science
Email: manucharova@mail.ru
Russian Federation, Moscow
A. V. Mardanov
Institute of Bioengineering, Research Center of Biotechnology
Email: manucharova@mail.ru
Russian Federation, Moscow
G. I. El’-Registan
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: manucharova@mail.ru
Russian Federation, Moscow
Supplementary files
