Determination of endoglucanase activity of paper decaying fungi from an old library at the ancient Medina of Fez


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Paper from an ancient library of the cultural city of Fez (Morocco) is exposed to rapid deterioration by variety of microorganisms, especially cellulolytic fungi. For this, ten isolates fungi previously isolated from historical biodeteriorated paper were screened for their ability to produce endoglucanase (CMCase), amylase, polygalacturonase and ligninase enzymes. The CMCase activity of cellulolytic strains was essayed in liquid media at 25°C for 10 days. Influence of temperature and pH were assessed for the production of CMCase by all the fungus isolated from decaying paper. The research findings from the present study demonstrate that all the tested isolates had cellulase, amylase, pectinase and ligninase activities. It was found that Mucor racemosus PF15, Aspergillus niger, and Aspergillus oryzae exhibited the maximum endoglucanase activity in liquid medium (0.256, 0.236, and 0.216 UI/mL in descending order) for six days. Temperature profiling revealed optimum endoglucanase activity at 25 and 30°C. Maximum activity was observed at pH 5 and pH 6.

作者简介

F. El Bergadi

Microbial Biotechnology Laboratory

Email: Ibnsoudas@yahoo.fr
摩洛哥, Fez

F. Laachari

Microbial Biotechnology Laboratory

Email: Ibnsoudas@yahoo.fr
摩洛哥, Fez

M. Sadiki

Microbial Biotechnology Laboratory

Email: Ibnsoudas@yahoo.fr
摩洛哥, Fez

S. Elabed

Microbial Biotechnology Laboratory; City of Innovation

Email: Ibnsoudas@yahoo.fr
摩洛哥, Fez; Fez

M. Iraqui

Microbial Biotechnology Laboratory

Email: Ibnsoudas@yahoo.fr
摩洛哥, Fez

S. Ibnsouda

Microbial Biotechnology Laboratory; City of Innovation

编辑信件的主要联系方式.
Email: Ibnsoudas@yahoo.fr
摩洛哥, Fez; Fez

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