Effect of mutations in extracellular nuclease on the characteristics of the pigmented and nonpigmented Serratia marcescens strains
- Authors: Nizamutdinova E.K.1, Shirshikova T.V.1, Mardanova A.M.1, Sharipova M.R.1, Bogomol’naya L.M.1
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Affiliations:
- Kazan Federal University
- Issue: Vol 85, No 1 (2016)
- Pages: 42-46
- Section: Experimental Articles
- URL: https://bakhtiniada.ru/0026-2617/article/view/162450
- DOI: https://doi.org/10.1134/S0026261716010069
- ID: 162450
Cite item
Abstract
Comparative characterization of the pigmented and nonpigmented Serratia marcescens strains and their extracellular nuclease mutants was carried out. Biomass accumulation by the mutant strains decreased on average by 20%, while proteolytic activity of the culture liquid was 4–5 times lower than in the case of the wild type strains. The mutants with impaired extracellular nuclease genes exhibited higher sensitivity to reactive oxygen species. Comparative analysis of motility of the strains revealed the highest flagellar activity in the wild type nonpigmented strain, while the cells of its mutant completely lost this feature.
Keywords
About the authors
E. Kh. Nizamutdinova
Kazan Federal University
Email: marsharipova@gmail.com
Russian Federation, Kazan
T. V. Shirshikova
Kazan Federal University
Email: marsharipova@gmail.com
Russian Federation, Kazan
A. M. Mardanova
Kazan Federal University
Email: marsharipova@gmail.com
Russian Federation, Kazan
M. R. Sharipova
Kazan Federal University
Author for correspondence.
Email: marsharipova@gmail.com
Russian Federation, Kazan
L. M. Bogomol’naya
Kazan Federal University
Email: marsharipova@gmail.com
Russian Federation, Kazan
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