Role of thiol redox systems in Escherichia coli response to thermal and antibiotic stresses
- Authors: Smirnova G.V.1, Lepekhina E.V.1, Muzyka N.G.1, Oktyabrsky O.N.1,2
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Affiliations:
- Institute of Ecology and Genetics of Microorganisms, Ural Branch
- Perm National Research Polytechnic University
- Issue: Vol 85, No 1 (2016)
- Pages: 23-32
- Section: Experimental Articles
- URL: https://bakhtiniada.ru/0026-2617/article/view/162444
- DOI: https://doi.org/10.1134/S0026261716010124
- ID: 162444
Cite item
Abstract
Isogenic knockout mutants of Escherichia coli deficient in components of the glutathione and thioredoxin redox systems and growing at various temperatures (20–46°C) exhibited considerable differences in growth rate and survival, as well as in expression of the antioxidant genes. In the parental strain E. coli BW25113 (wt) treated with ciprofloxacin, ampicillin, or streptomycin, dependence of survival on growth temperature was a V-shaped curve with the maximum sensitivity within the range corresponding to high growth rates (40–44°C). Significant inverse correlation was observed between log CFU at different temperatures and specific growth rate prior to antibiotic addition. This applied to most of the mutants, which exhibited higher resistance to the three antibiotics tested at nonoptimal temperatures (20 and 46°C) than at 37 and 40°C. No correlation was found between resistance to stress and antibiotics and expression of the antioxidant genes. The role of global regulators ppGpp and σs in E. coli resistance to antibiotics and nonoptimal temperatures was shown.
About the authors
G. V. Smirnova
Institute of Ecology and Genetics of Microorganisms, Ural Branch
Email: oktyabr@iegm.ru
Russian Federation, Perm
E. V. Lepekhina
Institute of Ecology and Genetics of Microorganisms, Ural Branch
Email: oktyabr@iegm.ru
Russian Federation, Perm
N. G. Muzyka
Institute of Ecology and Genetics of Microorganisms, Ural Branch
Email: oktyabr@iegm.ru
Russian Federation, Perm
O. N. Oktyabrsky
Institute of Ecology and Genetics of Microorganisms, Ural Branch; Perm National Research Polytechnic University
Author for correspondence.
Email: oktyabr@iegm.ru
Russian Federation, Perm; Perm
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