Expression of adipocytokine in heart fat depots depending on the degree of coronary artery atherosclerosis in patients with coronary artery disease
- 作者: Gruzdeva O.V.1, Dyleva Y.A.1, Belik E.V.1, Akbasheva O.E.1, Borodkinа D.A.1, Sinitsky M.Y.1, Naumov D.Y.1, Bychkova E.E.1, Fanaskova E.1, Palicheva E.I.1, Kuzmina A.A.1, Barbarash O.1
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隶属关系:
- Research Institute for Complex Issues of Cardiovascular Disease
- 期: 卷 76, 编号 2 (2021)
- 页面: 159-168
- 栏目: CARDIOLOGY AND CARDIOVASCULAR SURGERY: CURRENT ISSUES
- URL: https://bakhtiniada.ru/vramn/article/view/125661
- DOI: https://doi.org/10.15690/vramn1388
- ID: 125661
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Background. In coronary artery disease, a change in the adipocytokine content of local fat depots of the heart is observed. However, it has not yet been established whether the expression levels of the studied parameters are really related to the degree of atherosclerotic lesion of the spacecraft.
Aims — to identify the features of the expression of adiponectin, leptin and IL-6 by adipocytes of epicardial, perivascular and subcutaneous adipose tissue depending on the degree of atherosclerotic lesion of the coronary channel in coronary heart disease.
Materials and methods. The study conducted at the “Research Institute for Complex Issues of Cardiovascular Disease” in 2017–2020, included 84 patients with coronary heart disease (CAD), of which 39 with a moderate degree of atherosclerotic lesion of the coronary artery (CA) (≤ 22 points on the SYNTAX Score scale), 20 with severe (23–31 points) and 25 with extremely severe (≥ 32 points). Biopsies of subcutaneous (SAT), epicardial (EAT) and perivascular adipose tissue (PVAT) were obtained during elective coronary artery bypass grafting (CABG). The expression of adipocytokine genes was determined using polymerase chain reaction (real-time PCR) using TaqMan probes and the concentration of the studied adipocytokines in adipocyte culture medium by enzyme immunoassay. Statistical analysis was performed using Statistica 9.0, a one-dimensional and multi-dimensional logistic regression analysis.
Results. In CAD in adipocytes of the cardiac fat depot, a shift in the balance of adipocytokines is observed towards increased expression and secretion of leptin, IL-6 and a decrease in adiponectin with maximum manifestation in severe and extremely severe coronary lesions. Adipocytes of EAT were characterized by the minimal expression of the adiponectin gene against the background of the maximum — leptin and IL-6 in comparison with adipocytes SAT and PVAT.
Conclusions. Low expression of the adiponectin in EAT and PVAT against the background of increased expression of leptin and IL-6 is associated with an increase in the degree of atherosclerotic lesion of the coronary channel.
作者简介
Olga Gruzdeva
Research Institute for Complex Issues of Cardiovascular Disease
Email: o_gruzdeva@mail.ru
ORCID iD: 0000-0002-7780-829X
SPIN 代码: 4322-0963
MD, PhD
俄罗斯联邦, KemerovoYulia Dyleva
Research Institute for Complex Issues of Cardiovascular Disease
Email: dyleva87@yandex.ru
ORCID iD: 0000-0002-6890-3287
SPIN 代码: 2064-6262
MD, PhD
俄罗斯联邦, KemerovoEkaterina Belik
Research Institute for Complex Issues of Cardiovascular Disease
编辑信件的主要联系方式.
Email: sionina.ev@mail.ru
ORCID iD: 0000-0003-3996-3325
SPIN 代码: 5705-9143
Junior Research Associate
俄罗斯联邦, KemerovoOlga Akbasheva
Research Institute for Complex Issues of Cardiovascular Disease
Email: akbashoe@yandex.ru
ORCID iD: 0000-0003-0680-8249
SPIN 代码: 8042-6940
MD, PhD, Professor
俄罗斯联邦, KemerovoDaria Borodkinа
Research Institute for Complex Issues of Cardiovascular Disease
Email: alpheia@mail.ru
ORCID iD: 0000-0002-6221-3509
SPIN 代码: 8666-3500
MD, PhD, Senior Research Associate
俄罗斯联邦, KemerovoMaxim Sinitsky
Research Institute for Complex Issues of Cardiovascular Disease
Email: sinimu@kemcardio.ru
ORCID iD: 0000-0002-4824-2418
SPIN 代码: 1095-2352
Research Associate
俄罗斯联邦, KemerovoDanil Naumov
Research Institute for Complex Issues of Cardiovascular Disease
Email: naumdu@kemcardio.ru
ORCID iD: 0000-0002-3972-3551
SPIN 代码: 1087-1748
Laboratory Assistant
俄罗斯联邦, KemerovoEvgeniya Bychkova
Research Institute for Complex Issues of Cardiovascular Disease
Email: eugenia.tarasowa@yandex.ru
ORCID iD: 0000-0002-0500-2449
SPIN 代码: 8247-9881
Laboratory Assistant
俄罗斯联邦, KemerovoElena Fanaskova
Research Institute for Complex Issues of Cardiovascular Disease
Email: fanaskova70@mail.ru
ORCID iD: 0000-0003-2705-3252
SPIN 代码: 8829-7720
MD, PhD, Senior Research Associate
俄罗斯联邦, KemerovoElena Palicheva
Research Institute for Complex Issues of Cardiovascular Disease
Email: palichevaelena@rambler.ru
ORCID iD: 0000-0002-5642-7746
SPIN 代码: 6437-3222
MD, PhD, Senior Research Associate
俄罗斯联邦, KemerovoAnastasia Kuzmina
Research Institute for Complex Issues of Cardiovascular Disease
Email: stusha76@mail.ru
ORCID iD: 0000-0002-4807-7686
Scopus 作者 ID: 788345
MD, PhD, Professor, Corresponding Member of the RAS
俄罗斯联邦, KemerovoOlga Barbarash
Research Institute for Complex Issues of Cardiovascular Disease
Email: barb61@yandex.ru
ORCID iD: 0000-0002-4642-3610
SPIN 代码: 5373-7620
MD, PhD, Professor, Corresponding Member of the RAS
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