EPITHELIAL CELLS EXPRESSING TLR-2 LEVEL AND NOSE MUCOSA BIOCENOSIS IN ALLERGIC RHINITIS PATIENTS
- Authors: Tyurin Y.A1,2, Sharifullina AA1, Mustafin IG2, Fassakhov RS3
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Affiliations:
- Kazan Scientific Research Institute of Epidemiology and Microbiology
- Kazan State medical University
- Kazan State Medical Academy
- Issue: Vol 10, No 6 (2013)
- Pages: 20-24
- Section: Articles
- URL: https://bakhtiniada.ru/raj/article/view/120997
- DOI: https://doi.org/10.36691/RJA617
- ID: 120997
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Abstract
Background. Determination of local epithelial cells expressing TLR2 as an indicator of local immunity in allergic rhinitis (AR) patients with opportunistic species of staphylococci nasal mucosa colonization. Methods. Washed epithelium samples obtained from patients with seasonal AR (n=8) aged 19—42 years, and perennial AR (n=15) aged 19 —45 years, as well as a control group (20 patients) aged 19—45 years without allergic diseases were investigated. Epithelial cells expressing TLR2 receptors were determined by flow cytometry. Results. The level of epithelial cells expressing TLR2 receptor in patients with seasonal AR was almost in 1,9 times, in perennial AR group — in 1,7 times lower then in healthy individuals. In patients with perennial AR S. aureus was obtained in 96,0% (CI: 79,5—99,2), in association with Str. pyogenes in 29% (CI: 14,9—49,2), Neisseria spp. — in 63,0% (CI: 42,7—78,8). Seasonal allergic rhinitis was characterized by association of S. aureus and S. hemolyticus (70,0%, 44,4—85,8). Conclusion. Ratio of epithelial cells positive for TLR2 in nasal lavage from patients with AR was lower than in healthy volunteers. Indicators proportion of epithelial cells expressing TLR2 in nasal lavage in patients with seasonal AR during an exacerbation period was significantly reduced (1,7—1,9 times), in comparison with healthy volunteers. In AR patients with increased density of staphylococci strains in nasal mucosa increased local epithelial cells expressing TLR2 in nasal lavage was established.
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##article.viewOnOriginalSite##About the authors
Yu A Tyurin
Kazan Scientific Research Institute of Epidemiology and Microbiology; Kazan State medical University
Email: immunolab@yandex.ru
Russia
A A Sharifullina
Kazan Scientific Research Institute of Epidemiology and MicrobiologyRussia
I G Mustafin
Kazan State medical UniversityRussia
R S Fassakhov
Kazan State Medical AcademyRussia
References
- Ильина И.И. Аллергический ринит. Consilium medicum. 2000, № 2, с. 338-334.
- Шарифуллина А.А., Баязитова Л.Т., Клыкова Т. В. и соавт. Микрофлора полости носа у больных аллергическим ринитом. Нижегородский медицинский журнал. здравоохранение Приволжского федерального округа. 2004. Приложение, с. 56.
- Шарифуллина А.А. Сравнительная характеристика микробного биоценоза полости носа у здоровых и больных аллергическим ринитом детей. Мед. иммунол. 2006, № 2-3, с. 214.
- Караулов А.В., Алешкин В.А., Воропаева Е.А. и соавт. Показатели колонизационной резистентности слизистых ротоглотки как объективные критерии мукозального иммунитета при бронхитах у детей. Иммунология. 2012, № 5, с. 255-259.
- Метельская В. А., Алешкин В.А., Воропаева Е.А. и соавт. Колонизационная резистентность и иммунологическая реактивность слизистых ротоглотки у детей в норме и при бронхолегочных заболеваниях. вест. РАМН. 2010, № 7, с. 10-15.
- Hussein Y.M., Awad H.A., Shalaby S.M. et al. Toll-like receptor 2 and Toll-like receptor 4 polymorphisms and susceptibility to asthma and allergic rhinitis: a case-control analysis. Cell. Immunol. 2012, v. 274 (1-2), p. 34-38.
- Zhang G., Candelaria P., Mäkelä J.M. et al. Disparity of innate immunity-related gene effects on asthma and allergy on Karelia. Pediatr. Allergy Immunol. 2011, v. 22 (6), p. 621-630.
- Newcombe R.G. Two-sided Confidence Intervals for the Single Proportion: Comparison of Seven Methods. Statistics in Medicine. 1998, v. 17, p. 57-72.
- Толстопятова М.А., Буслаева Г.А., Козлов И.Г. Роль рецепторов врожденного иммунитета в развитии инфекционной патологии у новорожденных детей. Педиатрия. 2009, № 1, с. 115-119.
- Zang J.P., Yang C.C., Changes Y. Changes and clinical significance of Toll-like receptor 2 and 4 expression in neonatal infections. Zhonghua Er Ke Za Zhi. 2007, v. 45, p. 130-133.
- Bartemes K.R, Hirohito Kita. Dynamic role of epithelial-derived cytokines in asthma. Clin. Immunol. 2012, v. 143, p. 222-235.
- Chung F. Anti-inflammatory cytokines in asthma and allergy: interleukin-10, interleukin-12, interferon-gamma. Mediators Inflamm. 2001, v. 10, p. 51-59.
- Klimatsidas M., Anastasiadis K., Foroulis C. et al. Elevated levels of anti-inflammatory IL-10 and pro-inflammatory IL-17 in malignant pleural effusions. J. Cardiothorac. Surg. 2012, v. 4, p. 104.
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