No metabolites impact in allergic inflammation regulation and their contribution to remodelling bronchi mucous in bronchial asthma patients
- Authors: Ogorodova LM1, Kozina OV2, Gereng EA1, Ogorodova LM1, Kozina OV1, Gereng EA1
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Affiliations:
- Сибирский государственный медицинский университет
- Центр по профилактике и борьбе со СПИДом и инфекционных заболеваний
- Issue: Vol 6, No 5 (2009)
- Pages: 11-17
- Section: Articles
- URL: https://bakhtiniada.ru/raj/article/view/121268
- DOI: https://doi.org/10.36691/RJA1025
- ID: 121268
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Abstract
Material and methods. Morphological and morphometrical methods of investigation of bronchi mucous of 39 BA patients were used, IL-4, TNF-α, INF-g, IL-8, nitrite, 3-nitrotyrosine, nitrosoglutathion levels in bronchoalveolar washout were investigated, nitrite concentration in condensate of the exhaled air was measured. Results. The increased level of IL-4, TNF-α, INF-g, IL-8 in bronchoalveolar washout is associated with persistent Th2-inflammation. The BA severity correlate with highest IL-8, TNF-a, INF-g, nitrite and 3-nitrotyrosine level but with nitrosoglutathion deficit and in general is associated with structured architectonics bronchi mucous abnormalities.
Conclusion. The association of toxic NO metabolites, pro-inflammation cytokines levels, bronchi mucous path-omorphological abnormalities with clinical signs of BA confirms the role of toxic NO metabolites in development of Th2-inflammations.
About the authors
L M Ogorodova
Сибирский государственный медицинский университетСибирский государственный медицинский университет
O V Kozina
Центр по профилактике и борьбе со СПИДом и инфекционных заболеваний
Email: ovkozina2006@rambler.ru
Центр по профилактике и борьбе со СПИДом и инфекционных заболеваний
E A Gereng
Сибирский государственный медицинский университетСибирский государственный медицинский университет
L M Ogorodova
O V Kozina
E A Gereng
References
- Global Initiative for Asthma. NIH Publication number. 01-3659, NHLBI/WHO 2006. National Institutes of Health.
- Ricciardolo F.M., Sterk P.J., Gaston B. and Folkerts G. Nitric Oxide in Health and Disease of the Respiratory System. Physiol. 2004,v. 84, p. 731-765.
- Bove P.F., Vliet A. Nitric oxide and reactive nitrogen species in airway epithelial signaling and inflammation. Free Radic. Biol. and Med. 2006, v. 41 (4), p. 515-527.
- Giustarini D., Milzani A., Colombo R. et al. Nitric oxide and S-nitrosothiols in human blood. Clin. Chim. Acta 2003, v. 330 (1-2), p. 85-98.
- Truyen E., Coteur L., Dilissen E. Evaluation of airway inflammation by quantitative Th1/Th2 cytokine mRNA measurement in sputum of asthma patients. Thorax. 2006, v. 61 (3), p. 202-208.
- Romanska H.M., Polak J.M., Coleman R.A. et al. iNOS gene upregulation is associated with the early proliferative response of human lung fibroblasts to cytokine stimulation. J Pathol. 2002, v. 197, p. 372-379.
- Каминская Л.Ю., Жлоба А.А., Александрова Л.А. и др. Влияние донатора NO нитрозотиола глутатиона на уровень окислов азота и малонового диальдегида в крови крыс. Артериальная гипертензия. 2005, № 1, с. 10-17.
- Massey R.C., Dennis M.J., McWeeny D.J. Some observations concerning the S-nitroso and S-phenylsulphonyl derivatives of L-cysteine and glutathione. Tetrahedron Letters. 1985, v. 26 (16), p. 2013-2016.
- Barnes P.J. The role inflammation and anti-inflamatory medication in asthma. Respir. Med. 2002, v. 96, p. 9-15.
- Козина О.В., Огородова Л.М., Андрушкевич В.В. и соавт. Метаболиты оксида азота и их значение в патогенезе бронхиальной астмы. Клин. и лаб. диагн. 2008, №. 6, с. 34-37.
- Козина О.В., Андрушкевич В.В., Сазонов А.Э. и соавт. Клинико-биохимические аспекты развития обструкции бронхов при бронхиальной астме. Пульмонология. 2008, № 2, с. 52-57.
- Silvestri M., Bontempelli M., Giacomelli M. et al. High serum levels of tumour necrosis factor-a and interleukin-8 in severe asthma: markers of systemic inflammation? Clinic. Exp. Allergy. 2006, v. 36 (11), p. 1373-1381.
- Celio S., Troxler H., Durka S.S. et al. Free 3-nitrotyrosine in exhaled breath condensates of children fails as a marker for oxidative stress in stable cystic fibrosis and asthma. Nitric Oxide. 2006, v. 15 (3), p. 226-232.
- Старикова Э.А., Фрейдлин И.С., Соколов Д.И., Сельков С.А. Изменения свойств эндотелиальных клеток линии ЕА.hy 926 под влиянием фактора некроза опухоли α, интерферона-γ и интерлейкина-4. Иммунология. 2005, №. 2, с. 83-87.
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