Cultures of malarial plasmodium. V. V. Yakovlev (Leningrad Med. J., 1926, No. 10)

Abstract

The author tried to obtain these cultures according to Bass' and Johns' method, namely, after the seizure he took 10 cc of blood from v. mediana with a syringe, quickly transferred it to a graduated test tube with 0.1 50% dextrose at the bottom, and defibrinated by stirring with a glass rod. After defibrination, the stick and clot were removed, the test tube was sealed with a new sterile cotton plug, and placed in a thermostat at 40°-41° C. For further study, the author used red blood cells from the upper layer of sediment under the serum column - these red blood cells were carefully collected in a Pastenr tube and smears were made on slides.

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