About the precipitation reaction in trichinosis

Abstract

To obtain precipitating sera, rabbits were infected per os with trichins. The usual dose used was 250 to 1000 trichins in one or more doses. The exsanguination of rabbits was performed at different times, from the 3rd to the 26th day, and in one series of experiments even on the 50th day. At the same time, exsanguinated rabbits were examined to determine whether the infection had resulted in trichinosis. Sera of only those rabbits whose organs or intestines were found to contain trichins were tested. The antigen was prepared as follows: the muscles of rabbits infected with trichins were pulverized and then physiological solution with 0.4% pepsin solution and 0.25°/o hydrochloric acid was added.

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