Evaluation of the effectiveness of modified CRD tests in the diagnosis of preeclampsia
- 作者: Fedotov S.A.1,2,3, Gerasimova E.M.1, Vashukova E.S.2, Pakin V.S.2, Kapustin R.V.2, Pachuliya O.V.2, Glotov A.S.2, Kulichikhin K.Y.1, Chernoff Y.O.4, Rubel A.A.1
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隶属关系:
- St. Petersburg State University
- The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
- Pavlov Institute of Physiology
- Georgia Institute of Technology
- 期: 卷 71, 编号 4 (2022)
- 页面: 65-74
- 栏目: Original study articles
- URL: https://bakhtiniada.ru/jowd/article/view/109365
- DOI: https://doi.org/10.17816/JOWD109365
- ID: 109365
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BACKGROUND: Preeclampsia is a multisystem complication of pregnancy associated with an increased risk of maternal / perinatal morbidity and mortality. In this regard, the development and following improvement of low-cost and convenient methods for diagnosis of preeclampsia is essential for accurate prediction, quick confirmation of the diagnosis and convenient monitoring of the pathology.
AIM: The aim of this study was to optimize a preeclampsia diagnosis test system based on the binding of proteins to the Congo red dye (CRD test).
MATERIALS AND METHODS: The study used 70 urine samples obtained from patients diagnosed with preeclampsia (n = 25) and from non-preeclampsia pregnant women (n = 45). The samples were stained with Congo red and the dye retention in the sample on the membrane after washing was calculated. Before staining, protein concentrations in the urine samples were equalized using centrifugal concentrators or the samples were used with the original protein concentrations. To wash the samples from the unbound dye, either methanol or ethanol was used. To compare the effectiveness of four CRD test variants differing in sample preparation, staining, and washing, ROC analysis was performed (IBM SPSS Statistics 20 software).
RESULTS: The express CRD test was designed as an optimization of the conventional CRD test. The effectiveness of the express test (the area under the ROC curve being 0.9) was higher than that of the other three test options (the area under the ROC curve ranges from 0.67 to 0.82). The developed express CRD test can provide 95% specificity and 73% sensitivity, which indicates the promise of using this method in clinical diagnostics for the specific detection of preeclampsia patients.
CONCLUSIONS: Optimization of the CRD test has provided more effective protocols for diagnosis of preeclampsia from urine samples using Congo red (express CRD test) and has simplified the routine application of this test in clinical practice.
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作者简介
Sergey Fedotov
St. Petersburg State University; The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott; Pavlov Institute of Physiology
编辑信件的主要联系方式.
Email: serg900@yandex.ru
ORCID iD: 0000-0002-7428-120X
SPIN 代码: 8766-3012
Researcher ID: L-2467-2017
Cand. Sci. (Biol.)
俄罗斯联邦, Saint Petersburg; 3 Mendeleevskaya Line, Saint Petersburg, 199034; Saint PetersburgElizaveta Gerasimova
St. Petersburg State University
Email: elelovaya@gmail.com
ORCID iD: 0000-0003-1294-1919
M.Sc. (Mol. Med.), Research Assistant, The Laboratory of Amyloid Biology
俄罗斯联邦, Saint PetersburgElena Vashukova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: vi_lena@list.ru
ORCID iD: 0000-0002-6996-8891
SPIN 代码: 2811-8730
Researcher ID: D-3422-2017
Cand. Sci. (Biol.)
俄罗斯联邦, 3 Mendeleevskaya Line, Saint Petersburg, 199034Vladimir Pakin
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: pakins@list.ru
ORCID iD: 0000-0002-4358-8399
SPIN 代码: 4138-9973
MD, Obstetrician Gynecologist
俄罗斯联邦, 3 Mendeleevskaya Line, Saint Petersburg, 199034Roman Kapustin
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: kapustin.roman@gmail.com
ORCID iD: 0000-0002-2783-3032
SPIN 代码: 7300-6260
Researcher ID: G-3759-2015
MD, Dr. Sci. (Med.)
俄罗斯联邦, 3 Mendeleevskaya Line, Saint Petersburg, 199034Olga Pachuliya
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: for.olga.kosyakova@gmail.com
ORCID iD: 0000-0003-4116-0222
SPIN 代码: 1204-3160
MD, Cand. Sci. (Med.)
俄罗斯联邦, 3 Mendeleevskaya Line, Saint Petersburg, 199034Andrey Glotov
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: anglotov@mail.ru
ORCID iD: 0000-0002-7465-4504
SPIN 代码: 1406-0090
Researcher ID: E-8525-2015
Dr. Sci. (Biol.)
俄罗斯联邦, 3 Mendeleevskaya Line, Saint Petersburg, 199034Konstantin Kulichikhin
St. Petersburg State University
Email: konstantin_kulichikhin@yahoo.com
ORCID iD: 0000-0002-7443-4560
SPIN 代码: 6432-4970
Cand. Sci. (Biol.)
俄罗斯联邦, Saint PetersburgYury Chernoff
Georgia Institute of Technology
Email: yury.chernoff@biology.gatech.edu
ORCID iD: 0000-0002-8934-9051
SPIN 代码: 6201-0359
Researcher ID: J-2833-2014
School of Biological Sciences, Cand. Sci. (Biol.), Professor
美国, AtlantaAleksandr Rubel
St. Petersburg State University
Email: arubel@mail.ru
ORCID iD: 0000-0001-6203-2006
SPIN 代码: 3961-4690
Researcher ID: D-2903-2013
Cand. Sci. (Biol.)
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