Email this article Domestic devices for molecular genetic analysis: developments of the IAP RAS and SINTOL LLC
- Authors: Kurochkin V.E.1, Alekseev Y.I.2, Petrov D.G.1, Evstrapov A.A.1
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Affiliations:
- Institute for Analytical Instrumentation of the Russian Academy of Sciences
- “Syntol” LLC
- Issue: Vol 40, No 3 (2021)
- Pages: 69-74
- Section: Reviews
- URL: https://bakhtiniada.ru/RMMArep/article/view/76918
- DOI: https://doi.org/10.17816/rmmar76918
- ID: 76918
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Abstract
The set of domestic devices and reagent kits for molecular genetic analysis has been developed, which provides for all stages of the study, from the automated isolation of nucleic acids, their amplification with real-time detection to classical and genome-wide DNA sequencing. With the help of the created equipment and reagent kits, approaches for the specific indication of pathogens of a wide range of diseases have been successfully implemented. The high efficiency of the proposed approaches is shown by the example of detecting and analyzing the RNA of the pathogen SARS-CoV-2 in the fight against the COVID-19 coronavirus pandemic (7 figs, bibliography: 4 refs).
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##article.viewOnOriginalSite##About the authors
Vladimir E. Kurochkin
Institute for Analytical Instrumentation of the Russian Academy of Sciences
Email: lavrovas@yandex.ru
SPIN-code: 1868-9326
ResearcherId: S-7871-2016
D.Sc. (Тechnical), Professor
Russian Federation, 26, Rizhskiy ave., Saint Petersburg, 190103Yakov I. Alekseev
“Syntol” LLC
Email: jalex01@mail.ru
ORCID iD: 0000-0002-1696-7684
SPIN-code: 8145-5586
Ph.D. (Biology)
Russian Federation, MoscowDmitriy G. Petrov
Institute for Analytical Instrumentation of the Russian Academy of Sciences
Email: dimoon88@mail.ru
SPIN-code: 2975-8180
Scopus Author ID: 761722
Russian Federation, 26, Rizhskiy ave., Saint Petersburg, 190103
Anatoliy A. Evstrapov
Institute for Analytical Instrumentation of the Russian Academy of Sciences
Author for correspondence.
Email: an_evs@mail.ru
ORCID iD: 0000-0003-4495-8096
SPIN-code: 2053-5020
Scopus Author ID: 22691
ResearcherId: I-9739-2014
D.Sc. (Тechnical)
Russian Federation, 26, Rizhskiy ave., Saint Petersburg, 190103References
- Alekseev YaI, Belov YuV, Varlamov DA, et al. Devices for diagnostics of biological objects based on the method of polymerase chain reaction in real time (PCR-RT). Scientific Instrument Engineering. 2006;16(3):132–136. (In Russ.)
- Alekseev YaI, Belov YuV, Malyuchenko OP, et al. Genetic analyzer for DNA fragment analysis. Scientific Instrument Making. 2012;22(4):86–92. (In Russ.)
- Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome. Available from: https://www.ncbi.nlm.nih.gov/nuccore/NC_045512 (accessed 13.05.2021).
- Garafutdinov RR, Mavzyutov AR, Alekseev YaI, et al. Betacoronaviruses of humans and their highly sensitive detection using PCR and other methods of amplification. Biomics. 2020;12 (1):121–179. (In Russ.)
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