Email this article Receptor-binding domain of SARS-CoV-2 contribution to the neutrophil activation during 100 nm particle-induced immune response in conduction airway mucosa of mice
- Authors: Bolkhovitina E.L.1, Vavilova J.D.1, Bogorodskiy A.O.2, Okhrimenko I.S.2, Borshchevskiy V.I.2, Shevchenko M.A.1
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Affiliations:
- Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences
- Moscow Institute of Physics and Technology (National Research University)
- Issue: Vol 21, No 3 (2021)
- Pages: 97-102
- Section: Conference proceedings
- URL: https://bakhtiniada.ru/MAJ/article/view/79206
- DOI: https://doi.org/10.17816/MAJ79206
- ID: 79206
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Abstract
BACKGROUND: Airborne pathogens such as virus particles undergo elimination from the respiratory tract by mucociliary clearance and phagocytosis by immune cells. The data about phagocytic cell type infiltration and stimuli that attract phagocytic cells to conducting airway are required for the anti-virus immune response mechanism understanding and the treatment strategy development.
AIM: To detect the role of the receptor-binding domain of SARS-CoV-2 in neutrophil immune response activation in conducting airway mucosa after 100 nm particles application.
MATERIALS AND METHODS: C57BL/6 mice received an oropharyngeal application of fluorescent 100 nm particles suspended in the receptor-binding domain of SARS-CoV-2 solution. 24 hours after, conducting airways of mice were dissected and subjected for immunohistochemistry as whole-mounts. Three-dimensional images of conducting airway regions were obtained using confocal microscopy. Quantitative image analysis was performed to estimate the ingestion activity of neutrophils in conducting airway mucosa.
RESULTS: Neutrophil migration to conducting airway mucosa was detected in case of the application of particles in receptor-binding domain solution, but not in phosphate buffer or bovine serum albumin solution. Receptor-binding domain solution alone also induced neutrophil migration to conducting airway mucosa. Infiltrating conducting airway wall mucosa neutrophils contributed to particles internalization.
CONCLUSIONS: The receptor-binding domain of SARS-CoV-2 can activate the neutrophil-mediated response in conducting airway mucosa.
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##article.viewOnOriginalSite##About the authors
Elena L. Bolkhovitina
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences
Email: alenkash83@gmail.com
ORCID iD: 0000-0002-3386-509X
junior scientific researcher
Russian Federation, MoscowJulia D. Vavilova
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences
Email: Juliateterina12@gmail.com
ORCID iD: 0000-0002-9075-218X
Postgraduate student, junior scientific researcher
Russian Federation, MoscowAndrey O. Bogorodskiy
Moscow Institute of Physics and Technology (National Research University)
Email: bogorodskiy173@gmail.com
ORCID iD: 0000-0002-7589-7823
junior scientific researcher
Russian Federation, DolgoprudnyIvan S. Okhrimenko
Moscow Institute of Physics and Technology (National Research University)
Email: ivan.okhrimenko@phystech.edu
ORCID iD: 0000-0002-1053-2778
SPIN-code: 8418-0194
scientific researcher
Russian Federation, DolgoprudnyValentin I. Borshchevskiy
Moscow Institute of Physics and Technology (National Research University)
Email: borshchevskiy.vi@phystech.edu
ORCID iD: 0000-0003-4398-9712
SPIN-code: 2018-8957
senior scientific researcher
Russian Federation, DolgoprudnyMarina A. Shevchenko
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences
Author for correspondence.
Email: mshevch@gmail.com
ORCID iD: 0000-0001-5278-9937
SPIN-code: 8509-1096
scientific researcher
Russian Federation, MoscowReferences
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